Use of isotope ratio mass spectrometry to detect doping with oral testosterone undecanoate: Inter-individual variability of 13C/12C ratio
2006; Elsevier BV; Volume: 71; Issue: 5 Linguagem: Inglês
10.1016/j.steroids.2005.11.004
ISSN1878-5867
AutoresN Baume, C Saudan, Aurélien Desmarchelier, Emmanuel Strahm, Pierre‐Edouard Sottas, Carlo Bagutti, Michel Cauderay, Yorck Olaf Schumacher, Patrice Mangin, Martial Saugy,
Tópico(s)Forensic Toxicology and Drug Analysis
ResumoThe metabolic effect of multiple oral testosterone undecanoate (TU) doses over 4 weeks was assessed in seven voluntary men. The protocol was designed to detect accumulation of the substance by choosing the appropriate spot urines collections time and to study the urinary clearance of the substance after weeks of treatment. Urines were analysed by a new GC/C/isotope ratio mass spectrometry (IRMS) method to establish the delta(13)C-values of testosterone metabolites (androsterone and etiocholanolone) together with an endogenous reference compound (16(5alpha)-androsten-3alpha-ol). The significant differences in inter-individual metabolism following TU intake was illustrated by large variations in delta(13)C-values of both T metabolites (maximum Deltadelta(13)C-values = 5.5 per thousand), as well as by very stable longitudinal T/E profiles and carbon isotopic ratios in the first hours following administration. According to T/E ratios and delta(13)C-values, the washout period after 80 mg TU intake was less than 48 h for all subjects and no accumulation phenomenon was observed upon chronic oral administration.
Referência(s)