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Structural features and anticoagulant activities of a novel natural low molecular weight heparin from the shrimp Penaeus brasiliensis

1999; Elsevier BV; Volume: 1428; Issue: 2-3 Linguagem: Inglês

10.1016/s0304-4165(99)00087-2

1872-8006

Carl P. Dietrich, Jose F. de Paiva, Ricardo A.B. Castro, Suely F. Chavante, Walter Jeske, Jawed Fareed, Philip A.J. Gorin, Aline Mendes, Helena B. Nader,

Marine Sponges and Natural Products

A natural low molecular weight heparin (8.5 kDa), with an anticoagulant activity of 95 IU/mg by the USP assay, was isolated from the shrimp Penaeus brasiliensis. The crustacean heparin was susceptible to both heparinase and heparitinase II from Flavobacterium heparinum forming tri- and di-sulfated disaccharides as the mammalian heparins. 13C and 1H NMR spectroscopy revealed that the shrimp heparin was enriched in both glucuronic and non-sulfated iduronic acid residues. The in vitro anticlotting activities in different steps of the coagulation cascade have shown that its anticoagulant action is mainly exerted through the inhibition of factor Xa and heparin cofactor II-mediated inhibition of thrombin. The shrimp heparin has also a potent in vivo antithrombotic activity comparable to the mammalian low molecular weight heparins.