Electrochemiluminescence assay for the detection of acridinium esters
2002; Elsevier BV; Volume: 461; Issue: 1 Linguagem: Inglês
10.1016/s0003-2670(02)00254-4
ISSN1873-4324
AutoresMinli Yang, Chuanzhi Liu, Xinhui Hu, Pingang He, Yuzhi Fang,
Tópico(s)Antibiotics Pharmacokinetics and Efficacy
ResumoAn electrochemiluminescence (ECL)-based method for rapid and sensitive detection of acridinium ester in neutral solution was described. Strong ECL emission was observed when a positive voltage over 2.0 V (versus Ag/AgCl) was applied to the working electrode (Pt) immersed in the acridinium ester solution of 2.0 mol l−1 KNO3 (pH 7.0). The possible ECL mechanism was discussed. It was proposed that the ECL emission came out of N-methylacridone, the oxidization product of acridinium ester by the nascent oxygen generated on the surface of working electrode in the course of oxidization of water. Other influenced factors including the electrochemical parameters, the ECL reaction medium and pH value, were investigated in detail. Under the optimal conditions, ECL intensity has a linear relationship with the concentration of acridinium ester in the range of 0.24–96 ng ml−1 (r=0.9999). The relative standard deviation for 24 ng ml−1 acridinium ester was 4.6% (n=11). The limit of detection was 0.16 ng ml−1.
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