The micro-assay of intestinal disaccharidases
1976; Academic Press; Volume: 15; Issue: 2 Linguagem: Inglês
10.1016/0006-2944(76)90041-7
ISSN1557-7996
AutoresT. J. Peters, Roger M. Batt, James R. Heath, J. Tilleray,
Tópico(s)Enzyme Production and Characterization
ResumoThe sensitivity of Dahlqvist's method for the assay of intestinal disaccharidases has been enhanced at least 10-fold by the use of a fluorimetric assay for the liberated glucose. The amount of tissue is quantitated by a sensitive fluorimetric assay of its protein content. The normal range of the level of sucrase, maltase, and lactase in adult jejunum is recorded, and the enzyme activities in tissue from certain intestinal diseases are noted.
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