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Over Expression of Hypoxia-Inducible Protein 2, Hypoxia-Inducible Factor-1α and Nuclear Factor κB is Putatively Involved in Acquired Renal Cyst Formation and Subsequent Tumor Transformation in Patients With End Stage Renal Failure

2008; Lippincott Williams & Wilkins; Volume: 180; Issue: 2 Linguagem: Inglês

10.1016/j.juro.2008.04.006

1527-3792

Ryuichiro Konda, Jun Sugimura, Fumihiko Sohma, Toyomasa Katagiri, Yusuke Nakamura, Tomoaki Fujioka,

Mitochondrial Function and Pathology

No AccessJournal of UrologyAdult Urology1 Aug 2008Over Expression of Hypoxia-Inducible Protein 2, Hypoxia-Inducible Factor-1α and Nuclear Factor κB is Putatively Involved in Acquired Renal Cyst Formation and Subsequent Tumor Transformation in Patients With End Stage Renal Failure Ryuichiro Konda, Jun Sugimura, Fumihiko Sohma, Toyomasa Katagiri, Yusuke Nakamura, and Tomoaki Fujioka Ryuichiro KondaRyuichiro Konda , Jun SugimuraJun Sugimura , Fumihiko SohmaFumihiko Sohma , Toyomasa KatagiriToyomasa Katagiri , Yusuke NakamuraYusuke Nakamura , and Tomoaki FujiokaTomoaki Fujioka View All Author Informationhttps://doi.org/10.1016/j.juro.2008.04.006AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We examined hypoxia-inducible protein 2, hypoxia-inducible factor-1α and nuclear factor-κB in acquired cystic disease of the kidney associated with renal cell carcinoma to elucidate the roles of these factors in cyst formation and subsequent tumor transformation. Materials and Methods: Immunohistochemical expression of hypoxia-inducible protein 2, hypoxia-inducible factor-1α and phosphorylated nuclear factor-κB (active form) were examined in 20 normal kidney samples obtained from nephrectomy for localized renal cell carcinoma and 25 kidneys with acquired cystic disease associated renal cell carcinoma from 23 patients on dialysis. Results: Only faint or weak immunostaining for hypoxia-inducible protein 2, hypoxia-inducible factor-1α and phosphorylated nuclear factor-κB was observed in normal kidney tissues. In nontumor areas of the kidneys with acquired cystic disease expressions of these 3 proteins was up-regulated in tubular and cyst epithelial cells. Acquired cysts were classified into 3 types according to cyst epithelium morphology, namely flat, cuboidal and hyperplastic. Hyperplastic cysts were the predominant cysts expressing hypoxia-inducible protein 2 and hypoxia-inducible factor-1α. Although up-regulation of hypoxia-inducible protein 2, hypoxia-inducible factor-1α and phosphorylated nuclear factor-κB was observed in renal cell carcinoma, positive hypoxia-inducible protein 2 immunostaining was detected predominantly in papillary renal cell carcinoma, while positive hypoxia-inducible factor-1α and phosphorylated nuclear factor-κB immunostaining was prominent in clear cell renal cell carcinoma. Conclusions: Hypoxia-inducible protein 2, hypoxia-inducible factor-1α and phosphorylated nuclear factor-κB may be involved in a continuous process of the evolution of phenotypic expression from a simple cyst to epithelial hyperplasia and eventually to tumor. References 1 : Acquired cystic disease: mechanism and manifestation. Semin Nephrol1991; 6: 671. Google Scholar 2 : Renal neoplasm in acquired cystic disease. Am J Kidney Dis1995; 26: 1. 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Google Scholar Departments of Urology, Iwate Medical University School of Medicine, Morioka and Hachinohe City Hospital, Hachinohe and Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan© 2008 by American Urological AssociationFiguresReferencesRelatedDetails Volume 180 Issue 2 August 2008 Page: 481-485 Advertisement Copyright & Permissions© 2008 by American Urological AssociationKeywordskidneykidney failurecarcinoma, renal cellcystsHIG2 protein, humanMetrics Author Information Ryuichiro Konda More articles by this author Jun Sugimura More articles by this author Fumihiko Sohma More articles by this author Toyomasa Katagiri More articles by this author Yusuke Nakamura More articles by this author Tomoaki Fujioka More articles by this author Expand All Advertisement PDF downloadLoading ...