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Ca2+ stimulation of the external NADH dehydrogenase in Jerusalem artichoke (Helianthus tuberosum) mitochondria

1982; Elsevier BV; Volume: 109; Issue: 2 Linguagem: Inglês

10.1016/0006-291x(82)91751-x

1090-2104

Anthony L. Moore, Karl E.O. Åkerman,

Photoreceptor and optogenetics research

The stimulation of the external NADH dehydrogenase in Jerusalem artichoke (Helianthus tuberosum) mitochondria by Ca2+ has been investigated. By varying the extramitochondrial [Ca2+] between 10−7M and 10−3M using a Ca2+EGTA buffer system we find a considerable increase in NADH oxidation at micromolar free Ca2+ concentrations. The NADH induced mitochondrial membrane potential is reduced if the extramitochondrial Ca2+ is decreased to 10−7M. When [Ca2+] is increased to about 10−6M the membrane potential is restored. The results indicate that Ca2+ may be a modulator of the mitochondrial external NADH dehydrogenase in vivo.