Portal de Periódicos da CAPES

Human dendritic cells infected byListeria monocytogenes: induction of maturation, requirements for phagolysosomal escape and antigen presentation capacity

2000; Wiley; Volume: 30; Issue: 12 Linguagem: Inglês

10.1002/1521-4141(2000012)30

1521-4141

Annette Paschen, Kurt E.J. Dittmar, Roland Grenningloh, Manfred Rohde, Dirk Schadendorf, Eugen Domann, Trinad Chakraborty, Siegfried Weiß,

Listeria monocytogenes in Food Safety

European Journal of ImmunologyVolume 30, Issue 12 p. 3447-3456 ArticleFree Access Human dendritic cells infected by Listeria monocytogenes: induction of maturation, requirements for phagolysosomal escape and antigen presentation capacity Annette Paschen, Annette Paschen University Clinics Mannheim, Clinical Cooperation Unit for Dermato-Oncology of the DKFZ, Mannheim, GermanySearch for more papers by this authorKurt E. J. Dittmar, Kurt E. J. Dittmar GBF, German Research Centre for Biotechnology, Dept. of Molecular Biotechnology, Braunschweig, GermanySearch for more papers by this authorRoland Grenningloh, Corresponding Author Roland Grenningloh GBF, German Research Centre for Biotechnology, Molecular Immunology, Braunschweig, Germany Roland Grenningloh, Roche Milan Research, Via Olgettina 58, I-20132 Milan, Italy Siegfried Weiss, Molecular Immunology, GBF, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany Fax: +49–531–6181–444Search for more papers by this authorManfred Rohde, Manfred Rohde GBF, German Research Centre for Biotechnology, Dept. Microbiology, Braunschweig, GermanySearch for more papers by this authorDirk Schadendorf, Dirk Schadendorf University Clinics Mannheim, Clinical Cooperation Unit for Dermato-Oncology of the DKFZ, Mannheim, GermanySearch for more papers by this authorEugen Domann, Eugen Domann Institute for Medical Microbiology, University of Gießen, Gießen, GermanySearch for more papers by this authorTrinad Chakraborty, Trinad Chakraborty Institute for Medical Microbiology, University of Gießen, Gießen, GermanySearch for more papers by this authorSiegfried Weiss, Corresponding Author Siegfried Weiss siw@gbf.de GBF, German Research Centre for Biotechnology, Molecular Immunology, Braunschweig, Germany Roland Grenningloh, Roche Milan Research, Via Olgettina 58, I-20132 Milan, Italy Siegfried Weiss, Molecular Immunology, GBF, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany Fax: +49–531–6181–444Search for more papers by this author Annette Paschen, Annette Paschen University Clinics Mannheim, Clinical Cooperation Unit for Dermato-Oncology of the DKFZ, Mannheim, GermanySearch for more papers by this authorKurt E. J. Dittmar, Kurt E. J. Dittmar GBF, German Research Centre for Biotechnology, Dept. of Molecular Biotechnology, Braunschweig, GermanySearch for more papers by this authorRoland Grenningloh, Corresponding Author Roland Grenningloh GBF, German Research Centre for Biotechnology, Molecular Immunology, Braunschweig, Germany Roland Grenningloh, Roche Milan Research, Via Olgettina 58, I-20132 Milan, Italy Siegfried Weiss, Molecular Immunology, GBF, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany Fax: +49–531–6181–444Search for more papers by this authorManfred Rohde, Manfred Rohde GBF, German Research Centre for Biotechnology, Dept. Microbiology, Braunschweig, GermanySearch for more papers by this authorDirk Schadendorf, Dirk Schadendorf University Clinics Mannheim, Clinical Cooperation Unit for Dermato-Oncology of the DKFZ, Mannheim, GermanySearch for more papers by this authorEugen Domann, Eugen Domann Institute for Medical Microbiology, University of Gießen, Gießen, GermanySearch for more papers by this authorTrinad Chakraborty, Trinad Chakraborty Institute for Medical Microbiology, University of Gießen, Gießen, GermanySearch for more papers by this authorSiegfried Weiss, Corresponding Author Siegfried Weiss siw@gbf.de GBF, German Research Centre for Biotechnology, Molecular Immunology, Braunschweig, Germany Roland Grenningloh, Roche Milan Research, Via Olgettina 58, I-20132 Milan, Italy Siegfried Weiss, Molecular Immunology, GBF, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany Fax: +49–531–6181–444Search for more papers by this author First published: 22 November 2000 https://doi.org/10.1002/1521-4141(2000012)30:12 3.0.CO;2-MCitations: 40 The first two authors contributed equally to this work. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract An important feature of microbial infections is the ability of the microorganisms to interfere with and modulate the induction of host immune reactions. However, little is known about the effects of broad host range pathogens such as Listeria monocytogenes on similar cell types in different hosts. Here we examine the effects of the human and animal pathogen L. monocytogenes on human dendritic cells (DC) since this type of cells is essential for the initiation of immune responses. Listeria are phagocytosed efficiently by immature human DC and the bacteria escape from the phagolysosome quickly. Lack of the pore-forming activity of listeriolysin, which was found to be essential for the vacuolar escape of this bacterium in other cell types, retarded but did not prevent egress from the vacuole. Treatment of cultures of immature DC with L. monocytogenes resulted in rapid changes in morphology and cellular constitution followed by maturation of the DC. This could be judged by the appearance of maturation-specific cell surface markers. Antigen presentation to CD4 T cells was apparently not impaired by the infection. These results are in clear contrast to results obtained previously in the mouse system (Guzman et al., Mol. Microbiol. 1996. 20: 119 – 126; Darji et al., Eur. J. Immunol. 1997. 27: 1696 – 1703.). Citing Literature Volume30, Issue12December 2000Pages 3447-3456 ReferencesRelatedInformation