Neurotoxic esterase: Gel filtration and isoelectric focusing of carboxylesterases solubilized from hen brain
1984; Elsevier BV; Volume: 34; Issue: 12 Linguagem: Inglês
10.1016/0024-3205(84)90082-1
ISSN1879-0631
AutoresJörg-M. Chemnitius, Karl-H. Haselmeyer, Ronald Zech,
Tópico(s)Plant biochemistry and biosynthesis
ResumoCarboxylesterase activity (EC 3.1.1.1) of hen brain including neurotoxic esterases NTEA and NTEB is solubilized from lyophilized lipid-extracted brain material by the use of n-octylglucoside. The solubilized enzymes are subjected to free isoelectric focusing, six carboxyl - esterase activity peaks are obtained. By gel filtration on Sephacryl S-300 neurotoxic esterases are separated from carboxylesterase isoenzymes V and X. The molecular weight of the neurotoxic esterases is estimated to be 1.8 X 10(6).
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