Identification of enolase as a plasminogen‐binding protein in excretory–secretory products of Fasciola hepatica
2004; Wiley; Volume: 563; Issue: 1-3 Linguagem: Inglês
10.1016/s0014-5793(04)00306-0
ISSN1873-3468
AutoresDolores Bernal, José Enrique De la Rubia, Ana M Carrasco-Abad, Rafael Toledo, Santiago Más-Coma, Antonio Marcilla,
Tópico(s)Parasites and Host Interactions
ResumoWe have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host-parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identified the enolase enzyme, present in the excretory/secretory materials of F. hepatica, as a human plasminogen-binding protein. This enzyme has an apparent molecular weight of 47 kDa with pI ranging from 6.2 to 7.2. These results suggest that enolase could act as a plasminogen receptor.
Referência(s)