Portal de Periódicos da CAPES

Acute optic neuritis: Myelin basic protein and proteolipid protein antibodies, affinity, and the HLA system

1995; Wiley; Volume: 38; Issue: 6 Linguagem: Inglês

10.1002/ana.410380616

1531-8249

Finn Sellebjerg, Henrik Madsen, Jette Lautrup Frederiksen, Lars P. Ryder, Arne Svejgaard,

Systemic Lupus Erythematosus Research

Abstract Anti—myelin basic protein, anti—proteolipid protein, and anti—myelin basic protein peptide (amino acid residues 1–20, 63–88, and 89–101) antibody—secreting cells were studied in 20 patients with idiopathic optic neuritis, 20 with optic neuritis as part of multiple sclerosis, and 20 neurological control subjects. Antibody‐secreting cells were enumerated with an immunospot assay; the relative binding affinity of the antibodies was estimated by elution with thiocyanate. Patients with optic neuritis had more anti—myelin basic protein and anti—proteolipid protein antibodies than did control subjects (both p < 0.05); there was no difference between idiopathic optic neuritis and optic neuritis as a symptom of multiple sclerosis. Presence of the multiple sclerosis—associated DRB1 1501 gene was not associated with preferential synthesis of high‐affinity antibodies reactive with a single myelin basic protein peptide or with preferential synthesis of either anti—myelin basic protein or anti—proteolipid protein antibodies. The results demonstrate a potential for intrathecal synthesis of both anti—myelin basic protein and anti—proteolipid protein antibodies of high apparent affinity in patients with optic neuritis.