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RNASeq profiling of UTR expression during neuronal plasticity

2012; BioMed Central; Volume: 13; Issue: S12 Linguagem: Inglês

10.1186/1471-2105-13-s12-a4

1471-2105

Benjamin J. Harrison, Robert M Flight, Abdallah M. Eteleeb, Eric C. Rouchka, Jeffrey C. Petruska,

Cancer-related gene regulation

Background Neurons interact with, and are influenced by, tissues that are remote from the cell body. For example, sensory neuron cell bodies are located within peri-spinal ganglia but are connected to both the spinal cord and skin via their axons projecting through dorsal roots and peripheral nerves. Biochemical signals from anatomical compartments (spinal cord / root / ganglion / nerve / skin) modulate the molecular biology of neurons which can respond to signals from any/all of these remote regions. One mechanism by which neurons respond to these signals and interact with their targets is by actively transporting mRNA to that region. There, the mRNA is translated to produce protein at locally-determined positions and times. A growing body of evidence shows that untranslated regions (UTRs) of genes are important for this targeting. For example, 3’-UTRs contain 50nt “zip code” consensus binding sites for cis-acting zip code-binding proteins (ZPBs) that drive axonal targeting of mRNA [1]. We therefore hypothesized that gene expression during collateral sprouting, an axonal growth process that is highly responsive to target-derived factors, might involve differential regulation of UTR components.