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Correction: Proteomic Identification of Protein Targets for 15-Deoxy-Δ 12,14 -Prostaglandin J 2 in Neuronal Plasma Membrane

2011; Public Library of Science; Volume: 6; Issue: 4 Linguagem: Inglês

10.1371/annotation/ce01aaca-d54e-4042-9135-27d755605f4b

1932-6203

Yasuhiro Yamamoto, Kenkichi Takase, Junji Kishino, Megumi Fujita, Noboru Okamura, Toshiyuki Sakaeda, Masafumi Fujimoto, Tatsurou Yagami,

S100 Proteins and Annexins

15-deoxy-D 12,14 -prostaglandin J 2 (15d-PGJ 2 ) is one of factors contributed to the neurotoxicity of amyloid b (Ab), a causative protein of Alzheimer's disease.Type 2 receptor for prostaglandin D 2 (DP2) and peroxysome-proliferator activated receptorc (PPARc) are identified as the membrane receptor and the nuclear receptor for 15d-PGJ 2 , respectively.Previously, we reported that the cytotoxicity of 15d-PGJ 2 was independent of DP2 and PPARc, and suggested that 15d-PGJ 2 induced apoptosis through the novel specific binding sites of 15d-PGJ 2 different from DP2 and PPARc.To relate the cytotoxicity of 15d-PGJ 2 to amyloidoses, we performed binding assay [ 3 H]15d-PGJ 2 and specified targets for 15d-PGJ 2 associated with cytotoxicity.In the various cell lines, there was a close correlation between the susceptibilities to 15d-PGJ 2 and fibrillar Ab.Specific binding sites of [ 3 H]15d-PGJ 2 were detected in rat cortical neurons and human bronchial smooth muscle cells.When the binding assay was performed in subcellular fractions of neurons, the specific binding sites of [ 3 H]15d-PGJ 2 were detected in plasma membrane, nuclear and cytosol, but not in microsome.A proteomic approach was used to identify protein targets for 15d-PGJ 2 in the plasma membrane.By using biotinylated 15d-PGJ 2 , eleven proteins were identified as biotin-positive spots and classified into three different functional proteins: glycolytic enzymes (Enolase2, pyruvate kinase M1 (PKM1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH)), molecular chaperones (heat shock protein 8 and Tcomplex protein 1 subunit a), cytoskeletal proteins (Actin b, F-actin-capping protein, Tubulin b and Internexin a).GAPDH, PKM1 and Tubulin b are Ab-interacting proteins.Thus, the present study suggested that 15d-PGJ 2 plays an important role in amyloidoses not only in the central nervous system but also in the peripheral tissues.