Nucleotide sequence of Mec+ gene region of Streptomyces kasugaensis.
1985; Springer Nature; Volume: 38; Issue: 12 Linguagem: Inglês
10.7164/antibiotics.38.1795
ISSN1881-1469
AutoresKiyoshi Hirasawa, Masaru Ichihara, Masanori Okanishi,
Tópico(s)RNA and protein synthesis mechanisms
ResumoThe development of new host-vector systems in diverse species will be useful for breeding and gene cloning in Streptomyces.We have developed the host-vector system in Streptomyces kasugaensis, kasugamycin-and aureothricin-producer1,2). In the course of this study, DNA fragment containing Mec+ gene which restores the nutritional mutation of S. kasugaensis G3 requiring both methionine and cystein for its growth was inserted into pSK2, one of the plasmids resident in S. kasugaensis, and into pIJ7023), a plasmid of S. lividans, respectively.They were subcloned to elucidate the essential region of Mec+ gene
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