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Anti-type I Allergic Mechanisms of Mao-bushi-saishin-to in Mice

2000; Japanese Pharmacological Society; Volume: 82; Issue: 1 Linguagem: Inglês

10.1254/jjp.82.29

1347-3506

Yoshiki Ikeda, Akira Iizuka, Sakae Amagaya, Atsushi Ishige, Yasuhiro Komatsu, Eiichi Fujihira,

Asthma and respiratory diseases

We investigated the anti-allergic effect of mao-bushi-saishin-to (MBS) on the type I allergy model in mice. When MBS was administered orally at a dose of 0.5 or 1.0 g/kg, edema of the footpad, the amount of plasma IgE and the ratio of eosinophilic leukocytes in peritoneal exudate cells were all dose-relatedly suppressed. Moreover to investigate the anti-type I allergic mechanisms of MBS, enzyme-linked immunosorbent assay was performed to determine the interleukin (IL)-4, IL-5 and interferon (IFN)-gamma production from splenocytes that were stimulated by pokeweed mitogen for 48 h. In addition, we assayed IgE production from splenic B cells stimulated with the lipopolysaccharide and IL-4 for 7 days. MBS inhibited the IL-4 and IFN-gamma production, but IL-5 and IgE production were not affected. Thus possibly, the inhibition of IL-4 production may partially be involved in the expression of the anti-type I allergic effects of MBS.