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FRACTIONATION OF HABU SNAKE VENOM BY CHROMATOGRAPHY ON CM-CELLULOSE WITH SPECIAL REFERENCE TO BIOLOGICAL ACTIVITIES

1960; National Institute of Infectious Diseases; Volume: 13; Issue: 4-5-6 Linguagem: Inglês

10.7883/yoken1952.13.199

1884-2828

Akira Ohsaka,

Biochemical and Structural Characterization

One of the most prominent pathological changes evoked by the parenteral injection of Crotalidae venoms, including Habu venom, is hemorrhage (Taube et al., 1937; Fidler et al., 1940; Mitsuhashi et al., 1959; Kondo et al., 1960; Ohsaka et al., 1960 a, c) .Hemorrhage is claimed to be due, at least in part, to the action of proteolytic enzymes (Houssay, 1930; Kellaway, 1939; Zeller, 1948; Porges, 1953; van Heyningen, 1954; Slotta, 1955; Kaiser et al., 1958; Maeno et al., 1958) . Recently Ohsaka et al. (1960 a, b) reported that zone electrophoretic fractionation of Habu venom revealed the presence of at least two hemorrhagic principles, designated as HR1 and HR2, both of which were associated with proteolytic activity on casein.It is also well-known that Habu venom manifests lethal toxicity. It has been reported that the lethal toxicity of this venom was separable from such enzyme activities as phospholipase A, phosphodiesterase, 5'-nucleotidase and proteinase (with casein as substrate), which had been claimed to relate to the toxic actions (Ohsaka, 1958, 1960) . It is also interesting to show whether or not the principle responsible for the local effects also has the lethal toxicity. In our recent study it was indicated that one of the hemorrhagic principles (HR2) could be separated from at least the main part of lethal toxicity, while the other (HR1) associated with it (Ohsaka et al., 1960 a) .In the present work, a study was undertaken on chromatographic fractionation of venom of Habu (Trimeresurus flavoviridis), a Crotalidae, in an attempt to obtain further informations about the relationships among hemorrhagic activity, lethal toxicity and proteolytic activity.