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Delayed‐Type Hypersensitivity Skin Testing using Third Variable Loop Peptides Identifies T Lymphocyte Epitopes in Human Immunodeficiency Virus‐Infected Persons

1997; Oxford University Press; Volume: 176; Issue: 4 Linguagem: Inglês

10.1086/516517

1537-6613

Karl Sitz, Lawrence D. Loomis‐Price, Silvia Ratto‐Kim, Julie Kenner, Purnima Sau, Kenneth H. Eckels, Robert R. Redfield, Deborah L. Birx,

Food Allergy and Anaphylaxis Research

Cellular immune responses to human immunodeficiency virus type 1 (HIV-1) infection, particularly in vivo responses, have been difficult to study in large patient cohorts because of technical impediments. By use of small peptide fragments of the HIV-1 gp120 third variable loop, the CD4 T lymphocyte epitopes of 2 HIV-infected persons were mapped using a cutaneous delayed-type hypersensitivity (DTH) assay. The in vivo DTHresponses correlated with epitopes previously identified in vitro using CD4 T lymphocyte lines. The ability to determine CD4 T lymphocyte epitopes in large cohorts of patients using this simple in vivo technique would provide important diagnostic and prognostic data regarding effective immunoregulation of HIV-1. This technique should have broad applicability in HIV vaccine development and in the investigation of other immune-mediated human diseases.