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ELISA for toxoplasma antibody detection: a comparison with other serodiagnostic tests.

1980; BMJ; Volume: 33; Issue: 7 Linguagem: Inglês

10.1136/jcp.33.7.640

1472-4146

Andrea Balsari, G. Poli, V. Molina, M. Dovis, E. Petruzzelli, A. Boniolo, E. Rolleri,

Bacteriophages and microbial interactions

An ELISA method was developed for the measurement of toxoplasma IgG antibodies in human serum using antigen-coated polystyrene beads as a solid phase and anti human IgG-horse radish peroxidase conjugate as an enzymatic tracer. In order to assess ELISA sensitivity and specificity, a between methods comparison was made using 'conventional' serological tests as reference (dye-test, crossover-linked immunoassay, passive haemagglutination, indirect immunofluorescence). From an analysis of the group classifications obtained some considerations emerged: the ELISA specificity looks comparable with that of the 'reference' tests, as no sample classified as negative by all these tests was ELISA-positive, and vice versa; ELISA appears to correlate better with haemagglutination and immunofluorescence, on the basis of the respective class frequencies; in particular, the number of positives, which is much lower for the dye-test and crossover-linked immunoassay, suggests that a higher sensitivity is reached in the former cases.