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Estimation of β-structure content of proteins by means of deconvolved FTIR spectra

1985; Elsevier BV; Volume: 11; Issue: 4-5 Linguagem: Inglês

10.1016/0165-022x(85)90005-3

1872-857X

H. Susi, D. Michael Byler, James M. Purcell,

Molecular spectroscopy and chirality

Fourier self-deconvolution was applied to the infrared spectra of five globular proteins with a high beta-structure content and to the essentially alpha-helical protein hemoglobin. The featureless amide I' bands around 1650 cm-1 were thereby resolved into six to nine components, depending on the protein. Specific components were assigned to the beta-structure segments in each protein. The frequencies and the number of 'beta-bands' differ from one protein to another. The areas of the components were evaluated by means of a Gauss-Newton iteration procedure. It appears that the total area of the beta-bands, as a fraction of the total amide I' band area, reflects the relative beta-structure content of each protein studied.