Electrochemical fixation of carbon dioxide in pyruvic acid to yield malic acid using malic enzyme as an electrocatalyst
1990; Elsevier BV; Volume: 24; Issue: 2 Linguagem: Inglês
10.1016/0302-4598(90)85025-d
ISSN1872-8049
AutoresKenji Sugimura, Susumu Kuwabata, Hiroshi Yoneyama,
Tópico(s)Molecular Junctions and Nanostructures
ResumoElectrochemical reduction at a glassy carbon electrode of CO2-saturated Tris buffer solutions containing pyruvic acid, malic enzyme, nicotinamide adenine dinucleotide phosphate, ferredoxin-NADP+-reductase, and methyl viologen yielded malic acid as a result of fixation of CO2 in pyruvic acid. The net current efficiency was close to 100% under appropriate electrolysis conditions, and the turnover number for the active site-subunit of malic enzyme amounted to more than 20000 for an electrolysis for 18 h. The electron transfer from the electrode to methyl viologen seems to be the rate-determining step when the concentration of methyl viologen is less than 2 × 10−5 M, but above this concentration the rate-determining step is changed into the CO2-fixation reaction process at the malic enzyme.
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