Molecular-Based Methods for Quantifying HIV Viral Load
2004; Mary Ann Liebert, Inc.; Volume: 18; Issue: 2 Linguagem: Inglês
10.1089/108729104322802506
ISSN1557-7449
AutoresJames B. Peter, J. Sanders Sevall,
Tópico(s)HIV/AIDS Research and Interventions
ResumoViral load quantitation has become the major prognostic marker for disease prognosis and outcome of antiretroviral therapy in the treatment of HIV-infected individuals. The three major methodologies for viral load quantitation: the reverse transcriptase-polymerase chain reaction (RT-PCR; Amplicor™ HIV-1 Monitor Test, Roche Diagnostic Systems, Pleasanton, CA), the nucleic acid sequence-based amplification (NASBA; NucliSens™ HIV-1 QT Test, Organon Teknika, Bostel, The Netherlands); and a signal amplification methodology termed branchedchain DNA (bDNA) technique (Quantiplex™ HIV-1 RNA test, Bayer Diagnostics, Emeryville, CA) are briefly reviewed here.
Referência(s)