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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time

2007; Korean Society for Parasitology; Volume: 45; Issue: 4 Linguagem: Inglês

10.3347/kjp.2007.45.4.295

ISSN

1738-0006

Autores

Rosângela Soares Uzêda, Kattyanne de Souza Costa, Sara Lima Santos, Alexandre Moraes Pinheiro, Maria Ângela Ornelas de Almeida, Milton Μ. McAllister, Luís Fernando Pita Gondim,

Tópico(s)

Coccidia and coccidiosis research

Resumo

The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4℃, remain morphologically viable and infective to gerbils (Meriones unguiculatus). Six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. Two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for N. caninum at 1: 50 in an immunofluorescent antibody test. PCR using the brain from each gerbil did not reveal N. caninum specific DNA. We conclude that oocysts preserved for 46 mo are not infective, despite being morphologically intact. Key words: Neospora caninum, oocysts, Mongolian gerbils, Meriones unguiculatus, infectivity

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