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Comparison of proC and other housekeeping genes of Pseudomonas aeruginosa with their counterparts in Escherichia coli

1990; Elsevier BV; Volume: 86; Issue: 1 Linguagem: Inglês

10.1016/0378-1119(90)90121-7

1879-0038

Armand Savioz, David J. Jeenes, H.P. Kocher, D. Haas,

Molecular Biology Techniques and Applications

In a comparative study of housekeeping genes of Pseudomonas aeruginosa and Escherichia coli, the nucleotide sequence of a proline biosynthetic gene, proC, of P. aeruginosa has been determined. The subunit molecular mass (∼ 29 kDa) and the N-terminal amino acid sequence of purified Δ1-pyrroline 5-carboxylate reductase, the proC gene product, were in agreement with the proC nucleotide sequence. A survey of pairs of isofunctional genes from P. aeruginosa and E. coli reveals that within each pair, translated genes (including proC) have diverged more strongly than have untranslated genes specifying ribosomal or transfer RNAs. The translated genes, but not the untranslated ones, have a G+C content that is typical of the respective genomic G+C contents.