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Effect of Modification of the O-Methyltransferase Activity on Cell Wall Composition, Ultrastructure and Degradability of Transgenic Tobacco

1996; Wiley; Volume: 72; Issue: 3 Linguagem: Inglês

10.1002/(sici)1097-0010(199611)72

1097-0010

Marie Andrée Bernard Vailhé, Carole Migné, Agnès Cornu, Marie Paule Maillot, Elisabeth Grenet, Jean Michel Besle, Rossitza Atanassova, Françoise Martz, Michel Legrand,

Plant Gene Expression Analysis

Journal of the Science of Food and AgricultureVolume 72, Issue 3 p. 385-391 Research Article Effect of Modification of theO-Methyltransferase Activity on Cell Wall Composition, Ultrastructure and Degradability of Transgenic Tobacco Marie Andrée Bernard Vailhé, Marie Andrée Bernard Vailhé SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorCarole Migné, Carole Migné SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorAgnès Cornu, Agnès Cornu SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorMarie Paule Maillot, Marie Paule Maillot SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorElisabeth Grenet, Elisabeth Grenet SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorJean Michel Besle, Corresponding Author Jean Michel Besle SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorRossitza Atanassova, Rossitza Atanassova IBMP, CNRS, 12 rue Général Zimmer, 67084 Strasbourg, FranceSearch for more papers by this authorFrançoise Martz, Françoise Martz IBMP, CNRS, 12 rue Général Zimmer, 67084 Strasbourg, FranceSearch for more papers by this authorMichel Legrand, Michel Legrand IBMP, CNRS, 12 rue Général Zimmer, 67084 Strasbourg, FranceSearch for more papers by this author Marie Andrée Bernard Vailhé, Marie Andrée Bernard Vailhé SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorCarole Migné, Carole Migné SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorAgnès Cornu, Agnès Cornu SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorMarie Paule Maillot, Marie Paule Maillot SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorElisabeth Grenet, Elisabeth Grenet SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorJean Michel Besle, Corresponding Author Jean Michel Besle SRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSRNH, INRA de Clermont Ferrand-Theix, 63122 St Genès Champanelle, FranceSearch for more papers by this authorRossitza Atanassova, Rossitza Atanassova IBMP, CNRS, 12 rue Général Zimmer, 67084 Strasbourg, FranceSearch for more papers by this authorFrançoise Martz, Françoise Martz IBMP, CNRS, 12 rue Général Zimmer, 67084 Strasbourg, FranceSearch for more papers by this authorMichel Legrand, Michel Legrand IBMP, CNRS, 12 rue Général Zimmer, 67084 Strasbourg, FranceSearch for more papers by this author First published: November 1996 https://doi.org/10.1002/(SICI)1097-0010(199611)72:3 3.0.CO;2-LCitations: 42AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The effect of O-methyltransferase (OMT) cDNA modulation on cell wall composition, ultrastructure and rumen degradability was measured on transgenic tobacco (Nicotiana tabacum). The expression of OMT cDNA in antisense orientation (AS) inhibited OMT activity by 92% whereas expression of sense constructs led to plants either co-suppressed (CS, 98% inhibition) or overexpressing OMT activity. The cell wall residues of stems were analysed for lignin content, products of nitrobenzene oxidation (NBO) and polysaccharide content. Degradability was determined by a cellulase method. Sections of stem were stained by acid phloroglucinol and Mäule reactant. Stem samples were incubated in the rumen for 8, 24 and 48 h and observed by scanning electron microscopy (SEM). Compared to controls, OMT-depleted stems showed decreased hemicellulose content but unchanged lignin content. In contrast, syringyl units decreased by 40 and 90% in AS and CS samples respectively and NBO content followed a similar trend. Dry matter cellulase degradability was significantly improved by 3·5 and 5·6 percentage units in AS and CS samples respectively. SEM showed a greater bacterial colonisation in these samples and indicated a higher rate of rumen degradability in CS tissues than in controls. Overexpressing plants had a composition and a degradability similar to that of controls. For all the plants studied, the improvements in dry matter degradability were closely linked to the syringyl to guaiacyl ratio or to the NBO content. The modifications observed in down-regulated tobacco were similar to those produced by bm3 maize mutation, but without lignin decrease. Genetic modifications should therefore be considered for improving forage digestibility. Citing Literature Volume72, Issue3November 1996Pages 385-391 RelatedInformation