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Enhanced cell viability and cell adhesion using low conductivity medium for negative dielectrophoretic cell patterning

2010; Wiley; Volume: 5; Issue: 10 Linguagem: Inglês

10.1002/biot.201000194

1860-7314

Srinivasu Valagerahally Puttaswamy, Shilpa Sivashankar, Rong‐Jhe Chen, Chung‐Kuang Chin, Hwan‐You Chang, Cheng‐Hsien Liu,

Microbial Inactivation Methods

Abstract Negative dielectrophoretic (n‐DEP) cell manipulation is an efficient way to pattern human liver cells on micro‐electrode arrays. Maintaining cell viability is an important objective for this approach. This study investigates the effect of low conductivity medium and the optimally designed microchip on cell viability and cell adhesion. To explore the influence of conductivity on cell viability and cell adhesion, we have used earlier reported dielectrophoresis (DEP) buffer with a conductivity of 10.2 mS/m and three formulated media with conductivity of 9.02 (M1), 8.14 (M2), 9.55 (M3) mS/m. The earlier reported isotonic sucrose/dextrose buffer (DEP buffer) used for DEP manipulation has the drawback of poor cell adhesion and cell viability. A microchip prototype with well‐defined positioning of titanium electrode arrays was designed and fabricated on a glass substrate. The gap between the radial electrodes was accurately determined to achieve good cell patterning performance. Parameters such as dimension of positioning electrode, amplitude, and frequency of voltage signal were investigated to optimize the performance of the microchip.