Produção Nacional
Revisado por pares
Carbon sources effect on pectinase production from Aspergillus japonicus 586
2000; Springer Nature; Volume: 31; Issue: 4 Linguagem: Inglês
10.1590/s1517-83822000000400009
ISSN1678-4405
AutoresMaría Francisca Simas Teixeira, José Luiz Lima Filho, Nelsón Durán,
Tópico(s)Microbial Metabolites in Food Biotechnology
ResumoThe effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly affects the pectinesterase, and endo- and exopolygalacturonase activities. Pectin, glucose and saccharose, when added to the culture medium in high concentrations, exhibited a repression effect on all the analyzed enzymes.
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