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Starting at the Beginning, Middle, and End: Translation Initiation in Eukaryotes

1997; Cell Press; Volume: 89; Issue: 6 Linguagem: Inglês

10.1016/s0092-8674(00)80268-8

1097-4172

Alan B. Sachs, Peter Sarnow, Matthias W. Hentze,

Viral Infections and Immunology Research

In both prokaryotes and eukaryotes, ribosomes are recruited to mRNAs in a sequential, multistep process. In eukaryotes, following the recruitment of the small ribosomal subunit to the mRNA, the mRNA sequence is scanned and the small subunit is placed at the initiation codon. After this, the joining of the large ribosomal subunit to the mRNA completes the assembly of the ribosome. In spite of the similarities, there are large differences between prokaryotes and eukaryotes in how these essential features are enacted. In particular, while the recruitment of the small ribosomal subunit to mRNA in prokaryotes is primarily directed by the basepairing between the 16S rRNA and the Shine-Dalgarno sequence on mRNA, the recruitment of the small ribosomal subunit to mRNA in eukaryotes is primarily directed by protein–protein and protein–RNA interactions.