Effect of somatostatin on insulin secretion and cAMP content of isolated pancreatic rat islets
1978; Elsevier BV; Volume: 27; Issue: 9 Linguagem: Inglês
10.1016/0026-0495(78)90060-4
ISSN1532-8600
AutoresHans-Jürgen Hahn, Hubert Gottschling, P Woltanski,
Tópico(s)Diabetes Treatment and Management
ResumoIn a variety of endocrine glands and cells, somatostatin inhibits the release of peptide hormones. The inhibition of insulin and glucagon secretion has been uniformly reported in vivo as well as in vitro by using the perfused pancreas.1 With the collagenase-isolated islets, however, conflicting data concerning the effect of somatostatin on insulin2–7 and glucagon release3 have appeared. Therefore, we compared the effect of somatostatin on insulin secretion from islets prepared by microdissection or collagenase digestion in response to various initiators at concentrations that induce a maximal hormone secretion in islets from normal Wistar rats. Furthermore, since it has been suggested that the action of somatostatin on insulin secretion is mediated via cyclic adenosine-3′,5′-monophosphate (cAMP),2,4.6 the effect of somatostatin on islet cAMP content and the B cell response was investigated in the presence of stimulators of adenylate cyclase and inhibitors of phosphodiesterase.
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