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The Hitchhiker’s guide to Hi-C analysis: Practical guidelines

2014; Elsevier BV; Volume: 72; Linguagem: Inglês

10.1016/j.ymeth.2014.10.031

1095-9130

Bryan R. Lajoie, Job Dekker, N. Kaplan,

RNA and protein synthesis mechanisms

Over the last decade, development and application of a set of molecular genomic approaches based on the chromosome conformation capture method (3C), combined with increasingly powerful imaging approaches, have enabled high resolution and genome-wide analysis of the spatial organization of chromosomes. The aim of this paper is to provide guidelines for analyzing and interpreting data obtained with genome-wide 3C methods such as Hi-C and 3C-seq that rely on deep sequencing to detect and quantify pairwise chromatin interactions.