Adaptation of the Griess Reaction for Detection of Nitrite in Human Plasma
2004; Taylor & Francis; Volume: 38; Issue: 11 Linguagem: Inglês
10.1080/10715760400017327
ISSN1071-5762
AutoresDaniela Giustarini, Isabella Dalle‐Donne, Roberto Colombo, Aldo Milzani, Ranieri Rossi,
Tópico(s)Sulfur Compounds in Biology
ResumoAbstractThe determination of nitrite in human plasma or serum has been most frequently used as a marker of nitric oxide (NO) production. In addition, it has recently been suggested that nitrite could act as a vasodilating agent at physiological concentrations by NO delivery. Therefore, nitrite determination in biological fluids is becoming increasingly important. The most frequently used method to measure nitrite is based on the spectrophotometric analysis of the azo dye obtained after reaction with the Griess reagent. This method has some limitations regarding detection limit and sensitivity, thus resulting unsuitable for nitrite detection in plasma. We have identified some drawbacks and modified the original procedure to overcome these problems. By the use of the newly developed method, we measured 221±72 nM nitrite in human plasma from healthy donors.NitriteNitric oxideGriess reactionHuman plasma
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