Phospholipid asymmetry in renal brush-border membranes
1988; Elsevier BV; Volume: 942; Issue: 1 Linguagem: Inglês
10.1016/0005-2736(88)90285-4
ISSN1879-2642
AutoresCatherine Vénien‐Bryan, Christian Le Grimellec,
Tópico(s)Ion Transport and Channel Regulation
ResumoThe topological distribution of phospholipids between the inside and the outside of rabbit kidney brush-border membranes has been investigated by incubating membrane vesicles with sphingomyelinase, phospholipases A2 from bee venom and hog pancreas, phospholipases C and D, and trinitrobenzene sulfonate. Orientation and integrity of vesicles upon phospholipase treatment was determined by using two monoclonal antibodies recognizing an extracytoplasmic and a cytoplasmic domain, respectively, of the neutral endopeptidase (EC 3.4.24.11). It is shown that the transbilayer distribution of phospholipids is highly asymmetrical in kidney brush-border membranes: sphinomyelin accounted for 75% of the phospholipids present in the external leaflet, whereas phosphatidylethanolamine and phosphatidylserine plus phosphatidylinositol were found to comprise the majority of the inner layer of the membrane.
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