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Membrane on a Chip: A Functional Tethered Lipid Bilayer Membrane on Silicon Oxide Surfaces

2005; Elsevier BV; Volume: 89; Issue: 3 Linguagem: Inglês

10.1529/biophysj.105.061374

1542-0086

Vladimir Atanasov, Nikolaus Knorr, Randolph S. Duran, Sven Ingebrandt, Andreas Offenhäusser, Wolfgang Knoll, Ingo Köper,

Molecular Junctions and Nanostructures

Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as functionality of a bilayer membrane. Here, we describe the synthesis of lipids for a more modular design and the adaptation of the linker part to silane chemistry. We were able to form a functional tethered bilayer lipid membrane with good electrical sealing properties covering a silicon oxide surface. We demonstrate the functional incorporation of the ion carrier valinomycin and of the ion channel gramicidin.