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Biases in human papillomavirus genotype prevalence assessment associatedwith commonly used consensus primers

2005; Wiley; Volume: 118; Issue: 1 Linguagem: Inglês

10.1002/ijc.21299

1097-0215

Paul K.S. Chan, Tak‐Hong Cheung, Ann O. Y. Tam, Keith W.K. Lo, So‐Fan Yim, May M.Y. Yu, Ka‐Fai To, Yick‐Fu Wong, Jo L.K. Cheung, Denise Pui Chung Chan, Mamie Hui, Margaret Ip,

Cancer-related molecular mechanisms research

Abstract Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among squamous cell cervical cancer was underestimated from 14.6% to 0%. The fact that HPV52 should rank second in prevalence among squamous cell cervical carcinoma in Hong Kong could be missed if GP5+/6+, a worldwide commonly used primer set, was selected for HPV detection. Biases in HPV type‐specific sensitivity may result in misprioritization of vaccine candidates. © 2005 Wiley‐Liss, Inc.