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Direct electrogenerated chemiluminescence detection in high-performance liquid chromatography for determination of ofloxacin

2008; Elsevier BV; Volume: 623; Issue: 1 Linguagem: Inglês

10.1016/j.aca.2008.06.006

1873-4324

Yonghua Sun, Zhujun Zhang, Zhijun Xi,

Molecular Sensors and Ion Detection

Ofloxacin (OFLX) exhibited strong electrogenerated chemiluminescence (ECL) in NaNO3 solution with a dual-electrode system when constant current was exerted. Based on this observation, a sensitive direct ECL method coupled with high-performance liquid chromatography (HPLC) separation was developed for determination of OFLX in human serum. Factors affected the ECL emission were investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of OFLX in the range of 1.0 × 10−8 to 4.0 × 10−6 g mL−1 and the detection limit was 4 × 10−9 g mL−1 (S/N = 3). The proposed method was sensitive, simple and convenient to operate.