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Staphylococcus haemolyticus disseminated among neonates with bacteremia in a neonatal intensive care unit in Rio de Janeiro, Brazil

2013; Elsevier BV; Volume: 78; Issue: 1 Linguagem: Inglês

10.1016/j.diagmicrobio.2013.06.026

1879-0070

Paula Marcele Afonso Pereira, Vanessa Batista Binatti, Bruna Pinto Ribeiro Sued, Juliana Nunes Ramos, Renata Stavracakis Peixoto, Cláudio Simões, Eduardo Almeida de Castro, José Luís Muniz Bandeira Duarte, Verônica Viana Vieira, Raphael Hirata, Kátia Regina Netto dos Santos, Ana Luíza Mattos‐Guaraldi, José Augusto Adler Pereira,

Bacterial biofilms and quorum sensing

Oxacillin-resistant Staphylococcus haemolyticus (ORSH) was found as the most prevalent (77.5%) species of coagulase-negative staphylococci associated with bacteremia in neonates making use of intravenous catheters in an intensive care unit of a Brazilian teaching hospital. Thirty-one blood isolates were confirmed as S. haemolyticus by sequencing of the 16S and clustered in 6 pulsed-field gel electrophoresis types (with 58% of the strains belonging to 2 predominant types B and D). S. haemolyticus was mostly oxacillin-resistant (90.3%) displaying multiresistance profiles (70.4%). However, the mecA gene was undetected in 22.6% strains. ORSH exhibited slime production on Congo-Red agar (67.7%), adherence to polystyrene (96.7%), and glass (87%) surfaces. Interestingly, ica-operon was detected in 58% strains, mostly belonging to the B, D, and F genotypes, which is a significantly higher percentage when compared to other studies conducted at different parts of the globe. Data indicated that ica operon and biofilm-forming ORSH are endemic in Brazilian nosocomial environment.