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Identification of MIP-1α/LD78 as a Monocyte Chemoattractant Released by the HTLV-I-Transformed Cell Line MT4

1995; Mary Ann Liebert, Inc.; Volume: 11; Issue: 1 Linguagem: Inglês

10.1089/aid.1995.11.155

1931-8405

Riccardo Bertini, Walter Luini, Silvano Sozzani, Barbara Bottazzi, Paolo Ruggiero, Diana Boraschi, Daniela Saggioro, Luigi Chieco‐Bianchi, Paul Proost, Jo Van Damme, Alberto Mantovani,

Galectins and Cancer Biology

It is known that the HTLV-I-transformed cell line MT4 releases chemotactic activity for monocytes spontaneously. The MT4 monocyte chemoattractant was purified to homogeneity and sequencing of 25 amino acids revealed identity with the C-C chemokine macrophage inflammatory protein-1α (MIP-1α/LD78). An anti-MIP-1α/LD78 rabbit antiserum substantially inhibited chemotaxis of the MT4 chemoattractant. MT4 cells constitutively expressed MIP-1α/LD78 but not the C-C chemokines MCP-1, RANTES, and MIP-1β/Act2 and the C-X-C chemokines IL-8, groα, and groβ. MT4-derived MIP-1α/LD78 was active on monocytes but was a weak chemoattractant for polymorphonuclear leukocytes. Thus, MIP-1α/LD78 is a major monocyte chemoattractant released by HTLV-I-transformed T cells. Expression of MIP-1α/LD78, a leukocyte chemotactic and myelosuppressive molecule, may play an important role in the manifestations of HTLV-I-related diseases.