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Mycobacterium yongonense sp. nov., a slow-growing non-chromogenic species closely related to Mycobacterium intracellulare

2012; Microbiology Society; Volume: 63; Issue: Pt_1 Linguagem: Inglês

10.1099/ijs.0.037465-0

1466-5034

Byoung-Jun Kim, Renukaradhya K. Math, Che Ok Jeon, Hee-Kyung Yu, Young-Gil Park, Yoon‐Hoh Kook, Bum‐Joon Kim,

Infectious Diseases and Mycology

A slow-growing non-chromogenic mycobacterium was isolated from a patient with pulmonary disease. Phenotypically, strain 05-1390 T was similar to Mycobacterium intracellulare ATCC 13950 T . The 16S rRNA gene sequence (1385 bp) of strain 05-1390 T showed a high degree of similarity to those of the M. intracellulare complex, namely Mycobacterium marseillense 5351974 T (100 %), M. intracellulare ATCC 13950 T (99.8 %) and Mycobacterium chimaera DSM 44623 T (99.9 %). Phylogenetic analysis based on internal transcribed spacer 1 (ITS1) and the hsp65 gene indicated that strain 05-1390 T was closely related to M. intracellulare ATCC 13950 T , but that it was a distinct phylogenetic entity. Of particular interest, an analysis based on the rpoB gene (701 bp) showed that it is closely related to Mycobacterium parascrofulaceum ATCC BAA-614 T (99.4 %), a scotochromogenic strain, rather than to the M. intracellulare - related strains. Unique MALDI-TOF MS profiles also supported the taxonomic status of this strain as a distinct species. These data support the conclusion that strain 05-1390 T represents a novel mycobacterial species, for which the name Mycobacterium yongonense sp. nov. is proposed; the type strain is 05-1390 T ( = DSM 45126 T = KCTC 19555 T ).