Lysosomal enzyme activities in cultured lymphoid cell lines
1977; Elsevier BV; Volume: 80; Issue: 3 Linguagem: Inglês
10.1016/0009-8981(77)90141-3
ISSN1873-3492
AutoresIchiro Matsuda, Jiro Yamamoto, Nagta Noriyuki, Ninomiya Nobbuyuki, Izumi Akaboshi, Ohtsuka Hiroshi, Takato Katsuki,
Tópico(s)Biochemical Analysis and Sensing Techniques
ResumoSeveral lysosomal enzyme activities in cultured lymphoid cell lines were studied during 3 phases of cell culture; logarithmic growth phase, stationary phase and decline phase. Enzyme induction during cell growth was found in N-acetyl-hexosaminidase, beta-galactosidase and alpha-L-fucosidase, but no induction in alpha-D-mannosidase, alpha-glucosidase and beta-glucuronidase. The latter two enzymes were unchanged during all cell culture phases. A drop in alpha-L-fucosidase and alpha-D-mannosidase activity was found during the stationary and decline phases of cell culture.
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