Portal de Periódicos da CAPES

Identification of a site that mediates transcriptional response of the human β-actin gene to serum factors

1989; Elsevier BV; Volume: 75; Issue: 1 Linguagem: Inglês

10.1016/0378-1119(89)90378-8

1879-0038

Orita Satoshi, Makino Kozo, Kawamoto Takeshi, Hideo Niwa, Hirotaka Sugiyama, Takeo Kakunaga,

Genomics and Chromatin Dynamics

In quiescent fibroblasts, low-level transcription of the β-actin gene occurs, and it is rapidly induced by growth factors in serum. Deletion analysis of the 5' upstream region of the human β-actin gene using a CAT assay showed that the region between + 765 and + 783 downstream from the cap site, which had enhancer activity in growing NIH3T3 cells, also acted as a serum-response element in quiescent cells. The data suggest that the 19-bp element acts positively as a serum response element. This sequence contains a CC(A/T)6GG sequence which is found in the promoter region of many cytoskeletal and muscle-specific actin genes.