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BIBTEX RIS

Hedgehog signaling and osteoblast gene expression are regulated by purmorphamine in human mesenchymal stem cells

2011; Wiley; Volume: 113; Issue: 1 Linguagem: Inglês

10.1002/jcb.23345

1097-4644

Fabíola Singaretti Oliveira, L.S. Bellesini, Helton Luíz Aparecido Defino, Carlos Fernando Pereira da Silva Herrero, Márcio Mateus Beloti, Adalberto Luiz Rosa,

dental development and anomalies

Several biological events are controlled by Hedgehog (Hh) signaling, including osteoblast phenotype development. This study aimed at evaluating the gene expression profile of human mesenchymal stem cells (hMSCs) treated with the Hh agonist, purmorphamine, focusing on Hh signaling and osteoblast differentiation. hMSCs from bone marrow were cultured in non-osteogenic medium with or without purmorphamine (2 µM) for periods of up to 14 days. Purmorphamine up-regulated gene expression of the mediators of Hh pathway, SMO, PTCH1, GLI1, and GLI2. The activation of Hh pathway by purmorphamine increased the expression of several genes (e.g., RUNX2 and BMPs) related to osteogenesis. Our results indicated that purmorphamine triggers Hh signaling pathway in hMSCs, inducing an increase in the expression of a set of genes involved in the osteoblast differentiation program. Thus, we conclude that Hh is a crucial pathway in the commitment of undifferentiated cells to the osteoblast lineage.