Synergistic activation of intercellular adhesion molecule 1 (ICAM‐1) by TNF‐α and IFN‐γ is mediated by p65/p50 and p65/c‐Rel and interferon‐responsive factor Statlα (p91) that can be activated by both IFN‐γ and IFN‐α
1994; Wiley; Volume: 354; Issue: 2 Linguagem: Inglês
10.1016/0014-5793(94)01130-3
ISSN1873-3468
AutoresAndreas Jahnke, Judith P. Johnson,
Tópico(s)Immune Response and Inflammation
ResumoHuman ICAM‐1 expression is up‐regulated by IFN‐γ and TNF‐α, and synergistically increased by a combination of both. Transient expression of ICAM‐1/luciferase constructs led to definition of the regulatory regions mediating the cytokine response and showed that both are necessary for synergism. Immunochemical electromobility shift assays identified the TNF‐α‐dependent complexes that bound to the NF‐κB like sequence at −187 as p65/p50 and p65/c‐Rel. The interferon responsive region at −75 was bound by a Statlα (p91) containing complex that was activated by both IFN‐γ and IFN‐α. Although both regions were required for synergism, no additional or enhanced binding complexes were observed.
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