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U2 region of Epstein-Barr virus DNA may encode Epstein-Barr nuclear antigen 2.

1984; National Academy of Sciences; Volume: 81; Issue: 23 Linguagem: Inglês

10.1073/pnas.81.23.7632

1091-6490

T Dambaugh, Kathleen Hennessy, L Chamnankit, Elliott Kieff,

Cytomegalovirus and herpesvirus research

Sequence analysis of the U2 regions of the B95-8 and AG876 Epstein-Barr virus (EBV) isolates reveals divergence within a long open reading frame previously identified as encoding 1.5 kilobases of the 3' end of a viral RNA expressed in latently infected, growth-transformed, B-lymphocyte cell lines. Differences among EBV isolates within the U2 open reading frame are shown to correlate with differences in an EBV nuclear antigen, EBNA2. B95-8, W91, Raji, Cherry, and Lamont EBV isolates have similar U2 domains and encode similar-size EBNA2 proteins, while AG876, Jijoye, and P3HR-1 have variant or absent U2 domains and variant or absent EBNA2 proteins. The AG876 U2 open reading frame and EBNA2 protein are both shorter than those of B95-8. These data indicate that the U2 open reading frame encodes EBNA2.