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Presence of neutrophil-bearing antigen in lymphoid organs of immune mice

2006; Elsevier BV; Volume: 108; Issue: 9 Linguagem: Inglês

10.1182/blood-2006-04-016659

1528-0020

Belkys Maletto, Andrea S. Rópolo, Diego Alignani, Miriam V. Liscovsky, Romina P. Ranocchia, Víctor Gabriel Morón, María C. Pistoresi‐Palencia,

T-cell and B-cell Immunology

Abstract Neutrophils play a crucial early role during the innate response, but little is known about their possible contribution when an adaptive immune response is installed. A robust neutrophilia and a T helper 1 (Th1) immune response are present after immunization with Complete Freund Adjuvant (CFA). We show that when FITC-labeled OVA was injected into the footpad of OVA/CFA immunized mice, the main OVA-FITC+ cells recruited in draining popliteal lymph nodes (LNs) were neutrophils, with most of them arriving at the LN by means of lymphatic vessels. The development of this OVA-FITC+ neutrophil influx requires an immune response against OVA. The OVA-FITC+ neutrophils present in LNs displayed mainly intracellular TNF-α, and their depletion resulted in an increase in the specific IL-5 levels. These data provide new evidence about the role played by neutrophils in vivo in adaptive immunity.