Quantitation of aliphatic suberin in Quercus suber L. cork by FTIR spectroscopy and solid‐state 13 C‐NMR spectroscopy
2000; Wiley; Volume: 57; Issue: 6 Linguagem: Inglês
10.1002/1097-0282(2000)57
ISSN1097-0282
AutoresMarta Lopes, Carlos Pascoal Neto, António S. Barros, Douglas N. Rutledge, Ivonne Delgadillo, Ana M. Gil,
Tópico(s)Postharvest Quality and Shelf Life Management
ResumoThis work determined that the percentage of suberin in cork may be found by solid-state (13)C cross polarization/magic angle spinning (CP/MAS) NMR spectroscopy and by FTIR with photoacoustic detection (FTIR-PAS) spectroscopy. A linear relationship is found between the suberin content measured through CP/MAS spectral areas and that measured gravimetrically. Furthermore, application of a partial least squares (PLS1) regression model to the NMR and gravimetric data sets clearly correlates the two sets, enabling suberin quantification with 90% precision. Suberin quantitation by FTIR-PAS spectroscopy is also achieved by a PLS1 regression model, giving 90% accurate estimates of the percentage of suberin in cork. Therefore, (13)C-CP/MAS NMR and FTIR-PAS proved to be useful and accurate noninvasive techniques to quantify suberin in cork, thus avoiding the traditional time consuming and destructive chemical methods.
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