Identification of enzyme produced firefly oxyluciferin by reverse phase HPLC
2001; Elsevier BV; Volume: 42; Issue: 46 Linguagem: Inglês
10.1016/s0040-4039(01)01754-3
ISSN1873-3581
AutoresJoaquim C. G. Esteves da Silva, Júlia M. C. S. Magalhães, Rui Fontes,
Tópico(s)Biosensors and Analytical Detection
ResumoFirefly oxyluciferin (2-(6′-hydroxybenzothiazolyl)-4-hydroxythiazole) was chemically synthesized and characterized by means of 13C and 1H NMR, UV–vis spectrometry and RP-HPLC using different pH elution conditions. One of the chromatographic peaks observed in luciferase-catalyzed reaction mixtures was identified as corresponding to oxyluciferin.
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