Induction of a Sodium-dependent Depolarization by External Calcium Removal in Human Sperm
2003; Elsevier BV; Volume: 278; Issue: 38 Linguagem: Inglês
10.1074/jbc.m304479200
ISSN1083-351X
Autores Tópico(s)Plant Reproductive Biology
ResumoRemoval of external calcium with EGTA (from 2.5 mm to nanomolar levels) caused a remarkable depolarization in human sperm. This depolarization was initially fast. It was followed by a slow phase that brought the Vm to values of over 0 mV in 1–2 min. The slow and sustained phase correlated with a sustained decrease in intracellular calcium. However, calcium removal still induced depolarization in sperm with enhanced intracellular calcium (induced by progesterone), indicating that the sustained depolarization was not caused by a sustained intracellular calcium decrease. The depolarization was reduced as the external sodium content was substituted with choline, indicating that it was due to a sodium current, and was observed in lithium but not in tetramethylammonium-containing medium. In low sodium medium, the addition of sodium after calcium removal induced depolarization to the extent of which slightly increased in 2 min. The depolarization was completely inhibited by external magnesium (Ki = 1.16 mm). The addition of calcium or magnesium to calcium removal-induced depolarized sperm induced hyperpolarization that was inhibited by ouabain and was also prevented in medium without potassium, suggesting that the activity of the electrogenic Na+,K+-ATPase was involved. The conductance activated by calcium removal might unveil the presence of a calcium channel that in the absence of external calcium allows sodium permeation and that in normal conditions might contribute to the resting intracellular calcium concentration. Removal of external calcium with EGTA (from 2.5 mm to nanomolar levels) caused a remarkable depolarization in human sperm. This depolarization was initially fast. It was followed by a slow phase that brought the Vm to values of over 0 mV in 1–2 min. The slow and sustained phase correlated with a sustained decrease in intracellular calcium. However, calcium removal still induced depolarization in sperm with enhanced intracellular calcium (induced by progesterone), indicating that the sustained depolarization was not caused by a sustained intracellular calcium decrease. The depolarization was reduced as the external sodium content was substituted with choline, indicating that it was due to a sodium current, and was observed in lithium but not in tetramethylammonium-containing medium. In low sodium medium, the addition of sodium after calcium removal induced depolarization to the extent of which slightly increased in 2 min. The depolarization was completely inhibited by external magnesium (Ki = 1.16 mm). The addition of calcium or magnesium to calcium removal-induced depolarized sperm induced hyperpolarization that was inhibited by ouabain and was also prevented in medium without potassium, suggesting that the activity of the electrogenic Na+,K+-ATPase was involved. The conductance activated by calcium removal might unveil the presence of a calcium channel that in the absence of external calcium allows sodium permeation and that in normal conditions might contribute to the resting intracellular calcium concentration. Sperm of mammalian species present physiological changes that involve modulation of calcium entry mechanisms. In the female reproductive tract or in vitro in defined media, sperm undergoes in hours a series of complex biochemical changes known as "capacitation." Capacitation leads to changes in sperm motility patterns that requires calcium influx, resulting in an increment of ∼100 nm in resting intracellular calcium ([Ca2+] i) (1Baldi R. Casano C. Falsetti C. Krauz M. Forti G. J. Androl. 1991; 12: 323-330PubMed Google Scholar, 2Blackmore P.F. Cell Calcium. 1993; 14: 53-60Crossref PubMed Scopus (82) Google Scholar, 3Mendoza C. Tesarik J. FEBS Lett. 1993; 330: 57-60Crossref PubMed Scopus (47) Google Scholar). Only capacitated sperm bind to the zona pellucida and undergo the acrosome reaction (4Bleil J.D. Wassarman P.M. Dev. Biol. 1983; 95: 317-324Crossref PubMed Scopus (455) Google Scholar), an exocytotic process necessary for egg-sperm fusion. The acrosome reaction is physiologically induced by the egg zona pellucida glycoprotein ZP3, which triggers a rapid increase in [Ca2+] i that may involve the gating of voltage-dependent (VDCC) 1The abbreviations used are: VDCC, voltage-dependent calcium channels; SOC, store-operated calcium channels; diSC3(5), diisopropylthiodicarbocyanine iodide; H-HSM, Hepes-buffered human sperm medium; PTI, Photon Technology International; TMA, tetramethylammonium.1The abbreviations used are: VDCC, voltage-dependent calcium channels; SOC, store-operated calcium channels; diSC3(5), diisopropylthiodicarbocyanine iodide; H-HSM, Hepes-buffered human sperm medium; PTI, Photon Technology International; TMA, tetramethylammonium. and activation of store-operated (SOC) calcium channels (5Arnoult C. Kazam I.G. Visconti P.E. Kopf G.S Villaz M. Florman H.M. Proc. Natl. Acad. Sci. U. S. A. 1999; 96: 6757-6762Crossref PubMed Scopus (187) Google Scholar, 6O'Toole C.M. Arnoult C. Darszon A. Steinhardt R.A. Florman H.M. Mol. Biol. Cell. 2000; 11: 1571-1584Crossref PubMed Scopus (193) Google Scholar). Hence, it is evident that the study of the ion transport systems that set and regulate the membrane potential and the [Ca2+] i is necessary to fully understand the molecular basis of capacitation and acrosome reaction in human sperm. Unfortunately, the head of mature sperm is not suitable for patch clamp methods because of its particular shape and small size (7Darszon A. Labarca P. Nishigaki T. Espinoza F. Physiol. Rev. 1999; 79: 481-510Crossref PubMed Scopus (279) Google Scholar). Alternatively, the use of optical methods to detect membrane potential and intracellular ions have provided valuable information regarding the ion transport mechanism in mammalian sperm populations. The resting membrane potential values obtained with fluorescent probes in mouse and human sperm populations range between –60 and –20 mV (8González-Martínez M.T. Bonilla-Hernández M.A. Guzmán-Grenfell A.M. Arch. Biochem. Biophys. 2002; 408: 205-210Crossref PubMed Scopus (21) Google Scholar, 9Brewis I.A. Morton I, E. Mohammad S.N. Browes C.E. Moore H.D. J. Androl. 2000; 21: 238-249PubMed Google Scholar, 10Espinosa F. Darszon A. FEBS Lett. 1995; 372: 119-125Crossref PubMed Scopus (61) Google Scholar, 11Zeng Y. Clark E.N. Florman H.M. Dev. Biol. 1995; 171: 554-563Crossref PubMed Scopus (174) Google Scholar). Incidentally, it has been observed that in medium prepared without calcium, the resting membrane potential of human sperm populations have depolarized values as compared with those incubated in calcium-containing media (12Foresta C. Rossato F. DiVirgilio F. Biochem. J. 1993; 294: 279-283Crossref PubMed Scopus (101) Google Scholar, 13Guzmán-Grenfell A.M. Bonilla-Hernández M.A. González-Martínez M.T. Biochim. Biophys. Acta. 2000; 1464: 188-198Crossref PubMed Scopus (25) Google Scholar). In mouse sperm, the addition of calcium to sperm incubated in medium without calcium produces a transient plasma membrane hyperpolarization that is in part sensitive to the Na+,K+-ATPase inhibitor ouabain (10Espinosa F. Darszon A. FEBS Lett. 1995; 372: 119-125Crossref PubMed Scopus (61) Google Scholar). This evidence has led to the notion that calcium may regulate the resting membrane potential in mammalian sperm. In this paper, I report the effect of removing calcium from the medium and the subsequent calcium readdition on membrane potential and intracellular calcium in human sperm. It was possible to precisely compare membrane potential and internal calcium changes in human sperm population by using a simultaneous recording system (13Guzmán-Grenfell A.M. Bonilla-Hernández M.A. González-Martínez M.T. Biochim. Biophys. Acta. 2000; 1464: 188-198Crossref PubMed Scopus (25) Google Scholar, 14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar). It was found that removing calcium from the medium with EGTA produces a magnesium-sensitive, sodium-dependent depolarization in human sperm. This evidence suggests that sperm is endowed with calcium channels that in the absence of calcium allow sodium permeation. Materials and Media—Diisopropylthiodicarbocyanine iodide diSC3(5) was obtained from Molecular Probes. Fura-2/AM (acetoxymethyl ester), ionomycin, and valinomycin were from Sigma. The other reagents were obtained from Sigma, Merck, or Baker. Hepes-buffered human sperm medium (H-HSM), originally proposed by Suarez et al. (15Suarez S.S. Wolf D.P. Meizel S. Gamete Res. 1986; 14: 107-121Crossref Scopus (278) Google Scholar), had the following composition (in mm): 117.5 NaCl, 8.6 KCl, 2.5 CaCl2, 0.3 NaH2PO4, 0.49 MgCl2, 0.3 sodium pyruvate, 19 sodium lactate, 2 glucose, and 25 HEPES-Na (pH 7.6). H-HSM was modified so that NaCl was substituted with choline-Cl (lowNaH-HSM). This medium still contained ∼32 mm sodium (Na-Hepes, sodium lactate, sodium pyruvate, and NaH2PO4). Sperm Purification and Fura-2/AM Loading—Human semen was obtained from a panel of eight healthy 18–24-year-old donors. Normal samples were selected according to the World Health Organization protocol as reported previously (14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar). Sperm purification was performed using Percoll gradients according to Suarez et al. (15Suarez S.S. Wolf D.P. Meizel S. Gamete Res. 1986; 14: 107-121Crossref Scopus (278) Google Scholar) with minor modifications (14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar). Purified sperm (0.6–1.5 × 108 cells) were loaded with 2 μm Fura-2/AM in 2 ml of H-HSM medium at 36 °C for 40 min. Once washed, cells were incubated in 25 ml of H-HSM medium at 36 °C and immediately used for intracellular calcium and membrane potential measurements. Simultaneous Measurement and Calibration of [Ca2 + ]i and Membrane Potential—Membrane potential and [Ca2+] i were simultaneously detected with the fluorescent probes diSC3(5) and Fura-2/AM, respectively, with a PTI fluorometer (Photon Technology International) as reported previously (14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar). 2–5 ml of Fura-2/AM-loaded sperm (corresponding to ∼1 × 107 cells) was centrifuged at 300 × g for 5 min. The pellet (∼100 μl) was immediately added to the fluorescence cuvette containing 2.5 ml of H-HSM (or other) + 0.5 μm diSC3(5), previously thermostatized at 36 °C and under constant magnetic stirring. The fluorometer has two photomultipliers placed at 90° with respect to the Xenon lamp source. One photomultiplier detected the Fura-2/AM signal with a 488-nm filter (Andover), exciting at 340/380 with the excitation monochromator of the PTI system. The other photomultiplier detected the diSC3(5) fluorescence at 670 nm, exciting at 600 nm with a halide lamp placed in front of the xenon source. Both excitation and emission wavelengths for diSC3(5) measurements were achieved with optical interference filters (Andover). Data was acquired and digitalized at 0.83 Hz with the PTI interface system. Calibrations—The diSC3(5) fluorescence was calibrated in Fura-2/AM-loaded sperm suspensions as reported previously (14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar) with some modifications. At the end of each trace, 1.5 μm of the potassium ionophore valinomycin was added to bring the membrane potential to the Nernst potential value for potassium distribution (EK = –61.54 mV log [K]in/[K]ext). Taking into account that [K]in of human sperm is 120 mm (14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar) and T = 36 °C, the EK estimated for human sperm populations in H-HSM (K ext = 8.6 mm) is –71 mV. One minute after valinomycin, step depolarization to –43, –30, and –15 mV every 30 s was induced with consecutive additions of 15, 15, and 30 mm KCl. These depolarization concomitantly increased the fluorescence of diSC3(5) fluorescence. There is a linear relationship between the fractional change of diSC3(5) fluorescence (f = F – Fo /Fo , where Fo is the fluorescence value obtained upon valinomycin addition and F is the actual fluorescence value) and the membrane potential (16Wagoneer A.S. Annu. Rev. Biophys. Bioeng. 1979; 8: 47-68Crossref PubMed Scopus (414) Google Scholar) with a slope that in the sperm ranged between 0.018 and 0.025 (fractional change of fluorescence per millivolt). Taking the calibration parameters into account, the actual values of fluorescence were transformed into membrane potential values (Vm) according to Equation 1, Vm=F/mFo-1/m-b/m(Eq. 1) where m and b are the parameters of the linear calibration curve, i.e. the slope and the y axis value (fractional change of fluorescence) at 0 mV, respectively. These parameters were obtained by fitting linear the calibration data with a Microcal Origin, version 6.0 software. A calibration curve was made for each single trace using the step calibration described above with a maximal calibration value of –15 mV. However, the linear relationship is maintained up to +5 mV (range tested, data not shown). It should be noted that mitochondrial potential does not contribute to diSC3(5) signal in human sperm (13Guzmán-Grenfell A.M. Bonilla-Hernández M.A. González-Martínez M.T. Biochim. Biophys. Acta. 2000; 1464: 188-198Crossref PubMed Scopus (25) Google Scholar), which is consistent with a minor role of this organelle in supporting human sperm motility (17Hong C.Y. Chiang B.N. Wei Y.H. Br. J. Clin. Pharmacol. 1983; 16: 487-490Crossref PubMed Scopus (27) Google Scholar, 18Makler A. Makler-Shirane E. Stoller J. Lissak A. Abramovici H. Blumenfield Z. Arch. Androl. 1992; 29: 255-261Crossref PubMed Scopus (13) Google Scholar). Thus, simultaneous measurements of intracellular calcium and membrane potential were performed in the absence of mitochondrial inhibitors or uncouplers. The Fura-2/AM values were calibrated in H-HSM containing 500 nm diSC3(5) as reported previously (14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar). Experimental Assays—The effect of calcium removal from the medium with the calcium chelator EGTA on the intracellular calcium and membrane potential in human sperm was investigated. To conduct this test, 3 mm EGTA was added to the medium to decrease the 2.5 mm calcium present in H-HSM to nanomolar levels (see below). Because calcium chelation with EGTA produces proton release at an extent that can acidify the Hepes-buffered H-HSM medium, the concentrated EGTA solution (500 mm) was prepared in 3.2 m NaOH. In this condition, the addition of 3 mm EGTA to H-HSM did not produce any change in H-HSM pH (measured with a pH electrode). To estimate the calcium concentration in mixtures of EGTA-Ca in H-HSM and in H-HSM supplemented with magnesium, the calcium concentration calculator program Maxchelator (V.2.1) written by Chris Patton from the Hopkins Marine Station (Stanford University) was used. Statistical Analysis—Numeric results are expressed as the means ± S.E. n means number of individuals tested and analyzed with Student's t tests. Two-tailed p values < 0.05 were considered statistically significant. Fig. 1A shows the effect of 3 mm EGTA on the membrane potential and intracellular calcium detected simultaneously in human sperm. At this concentration, EGTA diminishes the extracellular calcium from 2.5 mm to ∼129 nm (see "Experimental Procedures"). The addition of EGTA caused a decrease in [Ca2+] i from an average of 220 ± 22 nm to 61 ± 6nm with a 50% decrease time of 8.7 ± 1.9 s (n = 8 individuals ± S.E.). Concomitantly, a remarkable depolarization of the plasma membrane was induced. The depolarization was initially fast and was followed by a large and slow phase that brought the membrane potential from resting –46 ± 3 to +18 ± 3.4 mV (n = 8, mean ± S.E.) in 1–2 min. This value remained nearly constant after a 10-min incubation in the fluorescence cell (data not shown). Once the membrane potential reached a constant value, 3 mm CaCl2 was added to restore the original calcium concentration. This addition produced an overshut in [Ca2+] i, i.e. a fast increase in [Ca2+] i that reached a peak in seconds at values above the resting internal calcium (495 ± 32 nm) and then was followed by a slow decrease that brought in ∼2 min the [Ca2+] i near to resting values. Concomitantly, calcium restoration produced a slow hyperpolarization that reached constant values in 2–3 min. In most cases, the membrane potential reached values more negative than resting (-66 ± 3.9 mV, n = 8, after 3 min of calcium addition), indicating that the membrane potential was not reversed to resting conditions. However, the mechanism involved in the depolarization induced by calcium removal was reversible. As shown in Fig. 1A, calcium removal induced by a further addition of 3.5 mm EGTA still induced depolarization that was again reversed by calcium readdition. The effect of EGTA was studied at different concentrations (Fig. 1B). The addition of 4.0, 3.0, and 2.75 mm EGTA that decreases the H-HSM external calcium concentration to approximately 41, 129, and 261 nm, respectively (see "Experimental Procedures"), caused in all of the cases sustained intracellular calcium decreases and sustained depolarization. These changes reached similar values, indicating that the effect of EGTA was saturating in both parameters. The addition of 2.0 and 2.25 mm EGTA that leaves ∼500 and 250 μm calcium in the medium produced small and fast transient depolarization with almost no change in internal calcium (Fig. 1B). The transient peaks slightly increased as the external calcium concentration decreased. The addition of 2.5 mm EGTA (that diminished calcium to 8 μm) produced in this case a large but transient depolarization, which was accompanied with a transient [Ca2+] i decrease, the depolarization phase was related to the decrease in [Ca2+] i and the hyperpolarization phase to [Ca2+] i recovery to resting values. This external calcium concentration (8 μm) seemed to be close to a critical concentration, which could lead to either a sustained or transient depolarization, as suggested by the fact that in some experiments 2.5 mm EGTA induced sustained depolarization. In these cases, the sustained depolarization was always accompanied by sustained decreases in [Ca2+] i (traces not shown). To explore whether the sustained depolarization was caused by the sustained [Ca2+] i decrease, the effect of calcium removal was studied in cells with a high [Ca2+] i content. To do this, a rise in [Ca2+] i was induced by progesterone (2Blackmore P.F. Cell Calcium. 1993; 14: 53-60Crossref PubMed Scopus (82) Google Scholar, 8González-Martínez M.T. Bonilla-Hernández M.A. Guzmán-Grenfell A.M. Arch. Biochem. Biophys. 2002; 408: 205-210Crossref PubMed Scopus (21) Google Scholar), and at the moment that [Ca2+] i reached enhanced values, calcium removal was induced. As shown in figure 2, calcium removal triggered depolarization at [Ca2+] i values as high as 400 nm that was reversed by calcium readdition. The depolarization progressed and reached sustained values as the [Ca2+] i decrease occurred even above resting values having 80% of the total depolarization when the decreasing [Ca2+] i reached resting values. This finding indicated that the sustained decrease in [Ca2+] i did not provoke by itself the sustained depolarization induced by calcium removal and supported the hypothesis that the whole depolarization is caused by calcium removal from external binding sites. The ion transport mechanism involved in the depolarization induced by calcium removal was studied by modifying the ionic composition of H-HSM. The total NaCl content was substituted with choline-Cl in H-HSM, a medium that still contains ∼51 mm sodium from 19 mm sodium lactate + ∼12.5 mm Na-Hepes + 0.3 mm NaHPO3 + 0.3 sodium pyruvate and ∼19 mm NaOH when the mixture EGTA-NaOH is added to the fluorescence cell (see "Experimental Procedures"). As shown in Fig. 3, A and B, in a wide range of sodium concentrations from 51 to 169 mm, the resting intracellular calcium and resting membrane potential values remained closed. In contrast, the EGTA-induced depolarization was remarkably diminished: the lower the external sodium, the lower the depolarization with no depolarization or slight hyperpolarization at 51 mm external sodium. This result indicated that the depolarization induced by calcium removal was attributed to a sodium current. In these experiments, the effect of calcium readdition is also shown. The smaller the sodium-dependent depolarization, the smaller the hyperpolarization induced by calcium restoration. It was noticeable that at low external sodium concentrations, calcium readdition produced a small transient depolarization. At high external [Na] (>70 mm), the hyperpolarization induced by calcium readdition seemed to hide the transient depolarization observed at low external [Na]. The calcium peak induced by calcium readdition in EGTA-treated sperm was smaller in low sodium medium as compared with normal H-HSM medium. To explore whether the sodium-dependent depolarization triggered by calcium removal inactivated or has inactivating components, the effect of sodium addition after calcium removal on the membrane potential was evaluated in lowNaH-HSM. Fig. 4 shows that 60 mm NaCl that was added 1 and 2 min after calcium removal with EGTA produced a depolarization that was similar in shape and in extent slightly bigger (120 ± 10%, n = 5, p < 0.05, after 2 min) than when the depolarization was triggered by EGTA in lowNaH-HSM medium supplemented with 60 mm NaCl. These experiments indicated that the sodium-dependent depolarization induced by calcium removal did not inactivate in at least 2 min in lowNaH-HSM but, conversely, a slight activation occurred. The selectivity of the sodium-dependent depolarization was studied. The effect of calcium removal induced by 3 mm EGTA was analyzed in lowNaH-HSM supplemented with 60 mm NaCl, LiCl, or TMA-Cl (tetramethylammonium chloride). As shown in Fig. 5, no depolarization was observed in medium with TMA. In contrast, in lithium containing medium, the depolarization was significantly (p < 0.05) larger (47 ± 4.0 mV, n = 4) than in sodium-containing medium (30 ± 3.8 mV). It was not possible to extend these studies to potassium, cesium, and rubidium since these cations caused depolarization of the membrane potential to values close to the Ek = –15 mV. It has been established that some calcium channels allow sodium influx in the absence of calcium that is blocked by magnesium (19Carbone E. Lux H.D. Carabelli V. Aicardi G. Zucker H. J. Physiol. (Lond.). 1997; 504: 1-15Crossref Scopus (46) Google Scholar, 20Minke B. Cook B. Physiol. Rev. 2002; 82: 429-472Crossref PubMed Scopus (526) Google Scholar). Thus, it was pertinent to explore whether the sodium-dependent depolarization induced by calcium removal was affected by magnesium. In the range of magnesium tested (up to 3 mm), the resting membrane potential, the resting [Ca2+]i, and particularly, the [Ca2+]i decrease induced by calcium removal were almost unaffected (Fig. 6A). However, increasing amounts of MgCl2 in the H-HSM medium caused a remarkable inhibition of the sodium-dependent depolarization induced by calcium removal with EGTA (Fig. 6A) with a Ki of 1.16 ± 0.12 mm (n = 5, mean ± S.E.) (Fig. 6B). Expectedly, magnesium was also able to reverse the depolarization induced by calcium removal (Fig. 6C). The hyperpolarization was dependent on the magnesium concentration and reached saturation at values close to those that produced blockade of the depolarization induced by calcium removal. Interestingly, the hyperpolarization was not related to any change in intracellular calcium. This suggested that at least a considerable extent of the hyperpolarization did not depend on the calcium influx or on the intracellular calcium increase that is induced by calcium addition in EGTA-treated sperm. The hyperpolarization induced by calcium and magnesium on calcium removal-induced depolarized sperm was studied. In mouse sperm, it has been observed that in calcium-deprived medium, calcium addition produce a transient hyperpolarization that is partially blocked by ouabain (10Espinosa F. Darszon A. FEBS Lett. 1995; 372: 119-125Crossref PubMed Scopus (61) Google Scholar). This led to the hypothesis that this hyperpolarization is in part related to the electrogenic 3Na+,2K+-ATPase activity. In human sperm, ouabain induces a slight depolarization in resting sperm (Fig. 7) (13Guzmán-Grenfell A.M. Bonilla-Hernández M.A. González-Martínez M.T. Biochim. Biophys. Acta. 2000; 1464: 188-198Crossref PubMed Scopus (25) Google Scholar) and no apparent effect on the depolarization was induced by calcium removal (Fig. 7). The hyperpolarization induced by calcium restoration and by magnesium was inhibited by ouabain, suggesting that the activity of a Na+,K+-ATPase contributed in a considerable extent to the hyperpolarization (Fig. 7). Consistently, the hyperpolarization was prevented on H-HSM prepared without potassium, a condition that stops the 3Na+,2K+-ATPase activity (21Robinson J.J. Flashner M.S. Biochim. Biophys. Acta. 1979; 549: 145-176Crossref PubMed Scopus (316) Google Scholar). Expectedly, the further addition of 1 mm KCl that should start the pump activity at saturating levels (21Robinson J.J. Flashner M.S. Biochim. Biophys. Acta. 1979; 549: 145-176Crossref PubMed Scopus (316) Google Scholar) caused hyperpolarization, the extent of which was similar to those observed in normal H-HSM (Fig. 7). In this paper, evidence is presented indicating that a remarkable depolarization carried out by sodium influx is triggered in human sperm when calcium is suddenly removed from the external medium. The depolarization has two apparent components. The initial one is fast, occurs in seconds, and is followed by the slow one that brings the membrane potential to values over 0 mV in 1–2 min. This permeability pathway remains open and is slightly stimulated in 2 min in lowNaH-HSM medium. It has a higher selectivity for lithium than for sodium and does not allow permeation of large monovalent cations like tetramethylammonium. The sodium-dependent depolarization values induced by calcium removal are far from the Nernst potential for sodium distribution (E Na). For instance, taking into consideration that [Na]in = 18 mm in human sperm (22Patrat C. Serres C. Jouannet P. Biol. Reprod. 2000; 62: 1380-1386Crossref PubMed Scopus (35) Google Scholar) at 121 mm external sodium, the E Na = +51 mV and the membrane potential reached upon calcium removal is ∼ –3 mV (Fig. 4). This indicates that the sodium-sensitive membrane potential value should result from the activation of the putative sodium current plus preexisting conductance that set the membrane potential in normal conditions and/or other conductance that is induced to buffer the depolarizing membrane potential. Potassium efflux though the activated permeability pathway could also explain why the sodium-dependent depolarization does not reach the E Na, even though potassium permeability should be considerably lower than that of sodium since the Ek = –71 mV. What kind of electrogenic sodium transporter may cause the depolarization induced by external calcium removal? Activation of an electrogenic 3Na+,1Ca2+ exchange should be discarded since experiments made in low sodium medium show that (a) the depolarization induced by sodium or lithium can be triggered in the absence of any change in [Ca2+] i , indicating that the depolarization occurs independently of changes in [Ca2+] i and (b) calcium removal decreases [Ca2+] i without affecting the membrane potential, indicating that the [Ca2+] i decrease may occur independently of the sodium-dependent depolarization. The most coherent hypothesis is that the sodium-dependent depolarization is caused by opening of sodium channels that are normally blocked by calcium ions. The simultaneous detection of intracellular calcium indicates that the initial fast component of the depolarization may occur before the [Ca2+] i starts to decrease. Indeed, this fast component may be triggered transiently when external calcium is suddenly lowered to the hundreds of micromolar range with EGTA at [Ca2+] i values still close to resting. The slow, large, and sustained depolarization is always accompanied by a slow [Ca2+] i decrease to levels below 100 nm and is induced when the external calcium concentration is lowered to the units of micromolar range being reproducibly observed in the hundreds of nanomolar range. In principle, the fact that a sustained [Ca2+] i decrease was always related to the sustained sodium-dependent depolarization suggested that there was an intracellular control site at [Ca2+] i with a Kd below 100 nm. However, the depolarization induced by calcium removal was observed even when the [Ca2+] i was enhanced with progesterone, indicating that the induction of depolarization is related to calcium removal from external binding sites. The inhibitory effect of magnesium on the depolarization induced by calcium removal and its ability to reverse the depolarization support the notion that the putative channel is controlled by divalent cation. Magnesium may act at the same level than calcium but with a much lower affinity (Ki = 1.16 mm). A sodium channel blocked in normal media seems to play no role in sperm physiology. Alternatively, the putative sodium channel may reveal the presence of a calcium channel in the plasma membrane that allows sodium permeation in the absence of external calcium. l-Type VDCC and transient receptor potential channels such as the SOCs allow large sodium currents when external calcium is brought to nanomolar and micromolar levels, respectively (19Carbone E. Lux H.D. Carabelli V. Aicardi G. Zucker H. J. Physiol. (Lond.). 1997; 504: 1-15Crossref Scopus (46) Google Scholar, 20Minke B. Cook B. Physiol. Rev. 2002; 82: 429-472Crossref PubMed Scopus (526) Google Scholar). In mature human sperm, there is a nifedipine-insensitive voltage-dependent calcium influx system, suggesting that these cells are endowed with VDCC (8González-Martínez M.T. Bonilla-Hernández M.A. Guzmán-Grenfell A.M. Arch. Biochem. Biophys. 2002; 408: 205-210Crossref PubMed Scopus (21) Google Scholar, 14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar). Molecular biology studies indicate that human sperm have a peculiar form of VDCC called cat sperm (23Ren D. Navarro B. Perez G. Jackson A.C. Hsu S. Shi Q. Tilly J.L. Clapham D.E. Nature. 2001; 413: 603-609Crossref PubMed Scopus (698) Google Scholar, 24Quill T.A. Ren D. Clapham D.E. Garbers D.L. Proc. Natl. Acad. Sci. U. S. A. 2001; 98: 12527-12531Crossref PubMed Scopus (240) Google Scholar). Even though these channels might allow sodium permeation in the absence of calcium, the sodium-dependent depolarization induced by calcium removal can be triggered even in hyperpolarized sperm (as shown in the second response in Fig. 1) in which VDCC should be mostly deactivated (14Linares-Hernández L. Guzmán-Grenfell A.M. Hicks-Gómez J.J. González-Martínez M.T. Biochim. Biophys. Acta. 1988; 1372: 1-12Google Scholar). Additionally, in some experiments, the depolarization was observed in sperm with resting membrane potential close to –15 mV in which VDCC should be inactivated (data not shown). This evidence indicates that depolarization is not caused by the opening of these channels. However, as sperm depolarizes (by calcium removal induction), the gating of VDCC and perhaps contribution to depolarization would be expected if these channels allow sodium to pass through. The acrosome vesicle is an internal store of calcium (25Walensky L.D. Snyder S.H. J. Cell Biol. 1995; 130: 857-869Crossref PubMed Scopus (234) Google Scholar, 26De Blas G. Michaut M. Trevino C.L. Tomes C.N. Yunes R. Darszon A. Mayorga L.S. J. Biol. Chem. 2002; 277: 49326-49331Abstract Full Text Full Text PDF PubMed Scopus (109) Google Scholar) that could activate a SOC channel (5Arnoult C. Kazam I.G. Visconti P.E. Kopf G.S Villaz M. Florman H.M. Proc. Natl. Acad. Sci. U. S. A. 1999; 96: 6757-6762Crossref PubMed Scopus (187) Google Scholar), which has been identified as a C2-type TRP protein with the use of antibodies in mouse sperm (27Junknickel M.K. Marrero H. Birnbaumer L. Lemos J.R. Florman H.M. Nature Cell Biol. 2001; 3: 499-502Crossref PubMed Scopus (277) Google Scholar). In cells suitable for patch clamp recordings, the sodium currents observed in the absence of external calcium could share some properties with the putative channel reported here. Indeed, in rat basophilic leukemia cells, SOC channels are sensitive to external magnesium in the millimolar range, allowing lithium permeation, but show similar selectivity for potassium and sodium (28Kozak J.A. Kerschbaum H.H. Cahalan M.D. J. Gen. Physiol. 2002; 120: 221-235Crossref PubMed Scopus (169) Google Scholar, 29Bakowski D. Parekh A.B. Pfluegers Arch. 2002; 443: 892-902Crossref PubMed Scopus (76) Google Scholar). In this study, SOC channel activation and contribution to the depolarization would be expected as a result of an intra-acrosomal calcium decrease imposed by calcium removal from external medium. However, the initial depolarization response induced by calcium removal in human sperm is too fast to be explained as a result of SOC activation. Nevertheless, SOC could activate later and contribute to the slow depolarization phase. The hypothesis that the sodium-dependent depolarization unveils the presence of a calcium channel has interesting consequences. In normal medium, the calcium influx through these channels must be so small that unlike sodium in the absence of calcium, no contribution to membrane potential is detected. Nevertheless, this calcium channel may have a major contribution to resting [Ca2+] i. This hypothesis implies that when calcium is removed from external binding sites, the calcium influx through the calcium channel is stopped and becomes a sodium-conducting channel depolarizing the cell. Consequently, the calcium-extruding mechanism, which must be permanently activated to control the resting [Ca2+] i , would cause the [Ca2+] i decrease. This decrease is possibly related to the activity of a Ca2+ATPase present in the plasma membrane that diminishes its own activity as [Ca2+] i decreases (30Edes I. Kranias E.G. Sperelakis N. Cell Physiology Source Book, Section II. Transport Physiology, Pumps and Exchangers. 2nd Ed. Academic Press, Orlando, FL1998: 225-236Google Scholar). When calcium is restored, the channel would switch its selectivity for calcium and allow calcium to pass through, increasing the [Ca2+] i. Because the calcium conductance through this channel would be much lower than sodium, no contribution to membrane potential would be expected. As for the effects of magnesium, the possibility that the putative channel allows magnesium permeation remains to be established. In any case, the hyperpolarization induced by calcium or by magnesium would be observed as a lack of contribution of the depolarizing sodium conductance to the membrane potential. The fact that the hyperpolarization is sensitive to ouabain and prevented in medium without potassium suggests that the activity of the 3Na+,2K+-ATPase is the main prevailing conductance that would hyperpolarize sperm by virtue of its electrogenic exchange. Interestingly, ouabain has a small depolarizing effect on resting membrane potential (Fig. 7) (13Guzmán-Grenfell A.M. Bonilla-Hernández M.A. González-Martínez M.T. Biochim. Biophys. Acta. 2000; 1464: 188-198Crossref PubMed Scopus (25) Google Scholar), suggesting that the 3Na+/2K+-ATPase does not importantly contribute to set it in resting condition. This suggests that as sperm depolarize (by calcium removal induction), the enzyme increases its activity possibly because of intracellular sodium loading (10Espinosa F. Darszon A. FEBS Lett. 1995; 372: 119-125Crossref PubMed Scopus (61) Google Scholar). This would explain why the Na+,K+-ATPase activity contributes at such a high extent to the membrane potential when magnesium or calcium is added to calcium removal-induced depolarized sperm. This mechanism agrees with that proposed for the transient hyperpolarization induced by calcium in calcium-deprived medium in mouse sperm (10Espinosa F. Darszon A. FEBS Lett. 1995; 372: 119-125Crossref PubMed Scopus (61) Google Scholar). A hyperpolarization dependent on the Na+,K+-ATPase activity has also been observed when glucose is added to human sperm incubated in medium without glucose (13Guzmán-Grenfell A.M. Bonilla-Hernández M.A. González-Martínez M.T. Biochim. Biophys. Acta. 2000; 1464: 188-198Crossref PubMed Scopus (25) Google Scholar). Besides the putative calcium channel, other mechanisms similar to SOC, VDCC, and/or the Na+/Ca+ exchanger might participate in the [Ca2+] i overshut induced by calcium readdition. The small and transient depolarization induced by calcium readdition in low sodium medium may indicate that indeed electrogenic calcium transport system(s) is involved. Accordingly, in mouse sperm incubated in medium prepared without sodium and calcium, calcium induces depolarization that is partially blocked by nimodipine, trifluoperazine, and magnesium, suggesting that this depolarization because of calcium influx through calcium channels (10Espinosa F. Darszon A. FEBS Lett. 1995; 372: 119-125Crossref PubMed Scopus (61) Google Scholar). In summary, this work shows evidence that suggests that human sperm is endowed with a magnesium-sensitive sodium conductance that is activated when external calcium is removed from the medium. This conductance may unveil the presence of calcium channels that might contribute to resting intracellular calcium. The physiological role of these putative channels, especially in sperm capacitation where regulation of the resting intracellular calcium is crucial, remains to be explored. I thank Esperanza Leos and Nicolas Alejandro Martínez Parra for the excellent technical assistance.
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