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Development and application of procedures for the highly sensitive quantification of cyclosarin enantiomers in hemolysed swine blood samples

2007; Elsevier BV; Volume: 859; Issue: 1 Linguagem: Inglês

10.1016/j.jchromb.2007.08.040

1873-376X

Georg Reiter, Marianne Koller, Horst Thiermann, Frédéric Dorandeu, John Mikler, Franz Worek,

Hormonal and reproductive studies

The present study was initiated to develop a sensitive method for the analysis of cyclosarin (O-cyclohexyl methylphosphonofluoridate, GF) enantiomers in biological samples utilizing classical configurations of GC–MS and automated solid phase extraction. To achieve this goal, a specific procedure had to be developed to extract cyclosarin from swine blood samples thereby stabilising and minimising the racemisation/deracemisation of its enantiomers. The chiral stationary phase was GAMMA DEX™ (gamma cyclodextrin), on which GF and deuterated GF enantiomers were baseline-resolved. The limit of detection was 1 pg for (−)-GF with GC–EI-MS and 5 pg for (+)-GF with GC–NCI-MS. The absolute recovery of the overall procedure for sample preparation was 85%. After an intravenous infusion of a supralethal dose of GF in anaesthetised swine only (−)-GF could be quantified, (+)-GF was not detected.