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Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study☆

2008; Elsevier BV; Volume: 875; Issue: 1 Linguagem: Inglês

10.1016/j.jchromb.2008.05.023

1873-376X

P DAFONSECA, Luís Alexandre Pedro de Freitas, Luís Felipe Ribeiro Pinto, Cezar Rangel Pestana, Pierina Sueli Bonato,

Plant Toxicity and Pharmacological Properties

An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively, and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY.