Cloning, expression, and nucleotide sequence of the alpha-amylase gene from the haloalkaliphilic archaeon Natronococcus sp. strain Ah-36
1994; American Society for Microbiology; Volume: 176; Issue: 16 Linguagem: Inglês
10.1128/jb.176.16.5131-5134.1994
ISSN1098-5530
AutoresTetsuo Kobayashi, Haruhiko Kanai, Rikizo Aono, Koki Horikoshi, Toshiaki Kudo,
Tópico(s)Genomics and Phylogenetic Studies
ResumoThe alpha-amylase gene of a Natronococcus sp. (1,512 bp) contained a signal peptide of 43 amino acids. Haloferax volcanii expressed the gene and cleaved the signal peptide accurately. The signal peptide shared an extremely high amino acid sequence identity with that of a protease from the halophilic archaeon 172P1.
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