Measuring Forces between Two Single Actin Filaments during Bundle Formation
2011; American Chemical Society; Volume: 11; Issue: 9 Linguagem: Inglês
10.1021/nl201630y
ISSN1530-6992
AutoresMartin Streichfuss, Friedrich Erbs, Kai Uhrig, Rainer Kurre, Anabel E.‐M. Clemen, Christian H. J. Böhm, Tamás Haraszti, Joachim P. Spatz,
Tópico(s)Force Microscopy Techniques and Applications
ResumoBundles of filamentous actin are dominant cytoskeletal structures, which play a crucial role in various cellular processes. As yet quantifying the fundamental interaction between two individual actin filaments forming the smallest possible bundle has not been realized. Applying holographic optical tweezers integrated with a microfluidic platform, we were able to measure the forces between two actin filaments during bundle formation. Quantitative analysis yields forces up to 0.2 pN depending on the concentration of bundling agents.
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