Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Graft copolymers with immobilized peroxidase for organic synthesis

1999; Elsevier BV; Volume: 55; Issue: 3 Linguagem: Inglês

10.1016/s0969-806x(98)00330-2

1879-0895

Alvaro Antonio Alencar de Queiroz, Reinaldo Romero Vargas, Olga Z. Higa, Élcio R. Barrak, Etelvino José Henriques Bechara, Blanka Wlasdislaw, Liliana Marzorati,

Biopolymer Synthesis and Applications

The graft copolymer poly(propylene)-graft-poly(acrylic acid) (PP-G-AA) was prepared by radiation-induced graft copolymerization of acrylic acid onto polypropylene spheres and characterized by thermal analysis and scanning electron microscopy (SEM). Maximum percentage of grafting (70%) was obtained at a total dose of 12 kGy using 30% (w/w) of acrylic acid. The Michaelis constant, KM, and the maximum reaction velocity, VMax, were determined for the free horseradish peroxidase and for the immobilized horseradish peroxidase. The enzyme affinity for the substrate (KM/Vmax) remains quite good after immobilization. The sulfoxidation reaction of a ketosulfide was investigated with the immobilized peroxidase.